湖南大学学报（自然科学版）2017，Vol.44Issue(6)：87-95,9.DOI:10.16339/j.cnki.hdxbzkb.2017.06.015
透明质酸改性PHBV组织工程纳米纤维支架的研究
Surface Modification of PHBV Tissue Engineering NanofibrousScaffolds with Hyaluronic Acid
摘要
Abstract
Surface properties of tissue engineering scaffold play important roles in cell adhesion and other biological processes such as cell proliferation,differentiation.In this study,the surface modification of Poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was performed in order to improve the biological activity of material surface.PHBV nanofibrous scaffold was firstly made by electrostatic spinning method,introducing free amino groups in the PHBV surface through 1,6-hexanediamine,which provided reaction sites for the further immobilization of hyaluronic acid (HA) macromolecules.The morphology observed by SEM showed that PHBV nanofibrous scaffolds prepared by electrostatic spinning method have smooth surfaces without obvious beads defects and their fiber diameters uniformly distribute.FTIR analysis proved that 1,6-hexanediamine and hyaluronic acid modified PHBV fibrous scaffolds were successfully achieved.The ninhydrin determination results showed that the surface amine group density,which increased with the extending of aminolysis time,reached its maximum at 50 minutes.Furthermore,there is no significant difference on the morphology of the scaffolds before and after modification by statistic comparison.The water contact angle significantly decreased after the immobilization of hyaluronic acid,indicating that the hydrophilicity was obviously improved after surface modification with the hydrophilic natural biological macromolecules.The Alamar Blue assay manifested that hyaluronic acid modified PHBV nanofibrous scaffolds could significantly promote the chondrocytes proliferation in vitro.Therefore,the hyaluronic acid modified PHBV nanofibrous scaffolds are potential for application in cartilage tissue engineering.关键词
透明质酸/PHBV纳米纤维支架/表面改性/亲水性/生物相容性Key words
hyaluronic acid/PHBV nanofibrous scaffolds/surface modification/hydrophilicity/biocompatibility分类
化学化工引用本文复制引用
刘海蓉,黄继英,周征,胡薏冰,李永生,邹鹏,戴瑶..透明质酸改性PHBV组织工程纳米纤维支架的研究[J].湖南大学学报（自然科学版）,2017,44(6):87-95,9.基金项目
教育部新世纪优秀人才(NECT-12-0170) （NECT-12-0170）
湖南省自然科学基金(2016JJ2023),Natural Science Foundation of Hunan Province(2016JJ2023) （2016JJ2023）
湖南省研究生创新基地(2014-2017) （2014-2017）
湖南大学交叉学科研究资助项目(2014JCA09) （2014JCA09）
