河南农业科学2017，Vol.46Issue(5)：77-83,7.DOI:10.15933/j.cnki.1004-3268.2017.05.014
番茄种质资源黄化曲叶病毒病抗性的鉴定与评价
Resistance Identification and Evaluation of Tomato Germplasm Resources to Tomato Yellow Leaf Curl Virus
摘要
Abstract
This study detected the resistance genes of tomato yellow leaf curl virus (TYLCV) in 86 tomato varieties by PCR amplification and identified their resistance to the viral disease in the field since it was difficult to identify the resistance of the present commercial tomato germplasms to TYLCV.The result showed that the resistance genes in tomato germplasms were mainly Ty-1/ty-1 and Ty-1/ty-1 + Ty-3a/ty-3,and the proportions of 2 types (19 and 23) in total resistant germplasms (61) were 31.15 ％ and 37.70％ respectively.Among the 25 tomato varieties which only contained Ty-1 gene,80.00％ of them performed resistance in the field.However,among the 26 tomato varieties which had both Ty-1 and Ty-3a genes,the resistance rate was 100％.It proved that the cumulative effect of resistance genes worked.Through the PCR technology to amplify the resistance genes,the disease-resistant detection accuracy was up to 81.40％.At the same time,we recommended 25 tomato varieties of resistance to TYLCV such as M-17,Dali and so on.关键词
番茄黄化曲叶病毒/Ty-1基因/Ty-2基因/Ty-3基因/抗性鉴定Key words
TYLCV/Ty-1/Ty-2/Ty-3/resistance identification分类
农业科技引用本文复制引用
米国全,陈梦莹,史艳艳,王裔娜,韩娅楠,程志芳,韩永平,王晋华..番茄种质资源黄化曲叶病毒病抗性的鉴定与评价[J].河南农业科学,2017,46(5):77-83,7.基金项目
河南省农业科学院自主创新专项基金项目(2016ZC24) （2016ZC24）
