河南农业科学2017，Vol.46Issue(5)：95-99,5.DOI:10.15933/j.cnki.1004-3268.2017.05.017
利用基于单链抗体的BIAcore技术检测蔬菜中的杀螟硫磷残留
Detection of Residual Fenitrothion in Vegetable Samples by BIAcore Based on Single Chain Antibody
摘要
Abstract
For establishing a method to fast measure residual fenitrothion in vegetables based on single chain antibody,the affinity and specificity of single chain variable fragments (ScFvs) against fenitrothion selected by ribosome display in our preliminary study were tested,and then a method for detection of residual fenitrothion in real vegetable samples by BIAcore X system based on the ScFvs was established.The results showed that three ScFvs of high affinity were obtained,the equilibrium dissociation constants with fenitrothion were 4.56 x 10-10,1.42 x 10-9,2.66 x 10-10 mol/L,respectively,and the cross-reactivity with other organophosphorus pesticides was below 0.1％ except for parathion-methyl(≤2.8％),which indicated that the ScFvs had a high specificity for fenitrothion.Recoveries of fenitrothion from fortified vegetables were in the range of 95.8％-102.2％ and the relative standard deviation(RSD) was below 2.13％.The results above indicated that this method was reliable,and could be used for fast measuring residual fenitrothion in real vegetable samples.关键词
杀螟硫磷/有机磷检测/单链抗体/BIAcoreKey words
fenitrothion/detection of organophosphorus/single chain antibody/BIAcore分类
农业科技引用本文复制引用
刘珊,罗义辉..利用基于单链抗体的BIAcore技术检测蔬菜中的杀螟硫磷残留[J].河南农业科学,2017,46(5):95-99,5.基金项目
国家质量监督检验检疫总局公益性行业科研专项(2012104003) （2012104003）
