河南农业科学2017，Vol.46Issue(5)：125-129,5.DOI:10.15933/j.cnki.1004-3268.2017.05.023
河南省及周边地区猪圆环病毒2型分子流行病学调查
Molecular Epidemiological Investigation of Porcine Circovirus Type 2 in Henan and Nearby Provinces
摘要
Abstract
In order to investigate molecular epidemiology of porcine circovirus type 2 (PCV2),a total of 158 samples including serum samples,lungs,lymph node were collected between 2014 and 2015 in Henan,Hebei,Shandong,Shanxi and Gansu provinces.All the 158 samples were subjected to PCR and the positive samples were sequenced to obtain the whole ORF2 gene.The results showed that 69 samples were PCV2 positive according to PCR,and 19 ORF2 genes were acquired.Sequence comparision showed that the nucleotide sequence homologies of ORF2 genes were up to 89.9％-99.9％,and the amino acid homologies were 88.0％-99.6％.The results indicated that the PCV2 was endemic in Henan province and provinces nearby,and PCV2 varied frequently.关键词
猪圆环病毒2型/ORF2/分子流行病学/序列分析/进化分析Key words
porcine circovirus type 2 (PCV2)/ORF2/molecular epidemiology/sequence analysis/evolutionary analysis分类
农业科技引用本文复制引用
焦文强,王治方,王克领,白献晓,郎利敏,李海利,朱文豪,张青娴,徐引弟..河南省及周边地区猪圆环病毒2型分子流行病学调查[J].河南农业科学,2017,46(5):125-129,5.基金项目
河南省科技攻关项目(162102110050) （162102110050）
