河南农业科学2017，Vol.46Issue(6)：98-103,124,7.DOI:10.15933/j.cnki.1004-3268.2017.06.018
寡糖对干旱胁迫下桔梗的诱导效应研究
Inductive Effect of Oligosaccharide on Platycodon grandiflorum under Drought Stress
摘要
Abstract
In order to study the drought resistance mechanism of oligosaccharide on Platycodon grandiflorum under drought stress,hydroponic experiments of Platycodon grandiflorum seed germination and pot experiments of Platycodon grandiflorum seedling were conducted,in which different concentrations of oligosaccharides(0,5,10,50 mg/L) were used to induce Platycodon grandiflorum under the PEG-6000(0,30,60,120 g/L) simulated drought stress,and the effects of oligosaccharides on seed germination,seedling antioxidant enzyme activity and MDA content of Platycodon grandiflorum under drought stress.The results showed that Platycodon grandiflorum seed germination indicators(germination potential,germination rate,germination index,root length and shoot length) enhanced first and then reduced with the increase of oligosaccharides concentration in a certain concentration range,under the same concentration of PEG-6000 simulated drought stress.At 10 mg/L of oligosaccharide Platycodon grandiflorum seed germination was the best.When Platycodon grandiflorum was induced by the same concentration of oligosaccharide,when the concentration of PEG-6000 was greater,the stress suffered by Platycodon grandiflorum seed was more serious.Germination potential,germination rate,germination index,root length and shoot length of Platycodon grandiflorum were positively correlated in different degrees.When Platycodon grandiflorum seedlings were induced by different concentrations of oligosaccharides(0,5,10,50 mg/L) under drought stress of 30 g/L PEG-6000,after 4-8 d,the activities of POD and CAT and content of MDA were increased at first and then decreased,the activity of SOD were raised gradually in Platycodon grandiflorum seedlings.When Platycodon grandiflorum seedlings were induced by 10 mg/L of oligosaccharide,the drought resistance result was the best.The activities of POD and CAT were highly significantly positively correlated(P<0.001).The activities of CAT and SOD were significantly positively correlated (P<0.05).The content of MDA in Platycodon grandiflorum seedlings was significantly negatively correlated with the activities of POD,CAT,SOD(P<0.01).关键词
桔梗/寡糖/干旱胁迫/抗旱性/生理指标Key words
Platycodon grandiflorum/oligosaccharide/drought stress/drought resistance/physical indicators分类
农业科技引用本文复制引用
李堆淑..寡糖对干旱胁迫下桔梗的诱导效应研究[J].河南农业科学,2017,46(6):98-103,124,7.基金项目
陕西省教育厅专项科研计划项目(16JK1239) （16JK1239）
商洛市科学研究项目(SK2015-12) （SK2015-12）
