河南农业科学2017，Vol.46Issue(6)：120-124,5.DOI:10.15933/j.cnki.1004-3268.2017.06.023
PRV感染PK-15细胞对prv-miR-LLT11a表达时相的影响
Effects on Temporal Expression of prv-miR-LLT11a in PK-15 Cells Infected by Pseudorabies Virus
摘要
Abstract
In order to study the pathogenic mechanism of pseudorabies virus(PRV),PK-15 cells were infected with PRV QBA strain at 0.5 MOI,and cells were harvested and microRNA(miRNA) were extracted at 0,1,2,4,6,8,12,24 hours post infection(hpi).Then the temporal expression of prv-miR-LLT11a was detected by stem-loop real-time quantitative PCR(RT-qPCR).The amplification results of RT-qPCR showed that the melting curve had a single peak and a clear inflection point.RT-qPCR data showed that the expression of prv-miR-LLT11a remarkably significant increased in PK-15 cells after 1 hour infection with PRV QBA strain,then decreased.Its expression level decreased to the lowest level at 6 hpi,and increased significantly after 8 hpi.关键词
伪狂犬病病毒/prv-miR-LLT11a/茎环RT-qPCR/表达时相Key words
PRV/prv-miR-LLT11a/stem-loop RT-qPCR/temporal expression分类
农业科技引用本文复制引用
杨利,石昂,李新果,李双双,时庆贺,郑关民,王玉国,陈陆,王川庆..PRV感染PK-15细胞对prv-miR-LLT11a表达时相的影响[J].河南农业科学,2017,46(6):120-124,5.基金项目
国家自然科学基金项目(31272567) （31272567）
河南省高校科技创新人才支持计划项目(14HASTIT022) （14HASTIT022）
河南省高校科技创新团队支持计划项目(14IRTSTHN015) （14IRTSTHN015）
