湖南中医药大学学报2017，Vol.37Issue(7)：719-722,4.DOI:10.3969/j.issn.1674-070X.2017.07.006
基于RAGE/NF-κB/mTOR信号通路探讨滋阴活血解毒方对AGEs诱导VEC损伤的干预作用
Effect of Ziyin Huoxue Jiedu Decoction on VEC Injury Induced by AGEs Based on RAGE / NF-κB / mTOR Signaling Pathway
摘要
Abstract
Objective To observe the effect of Ziyin Jiedu decoction on the damage of human umbilical vein endothelial cell (HUVEC) induced by AGEs and the change of RAGE/NF-κB/mTOR pathway, and to explore the protective effect of this prescription on AGEs-induced VEC injury. Methods The damage of HUVEC cells were induced by 100 mg/mL AGEs and randomly divided into seven groups. Three of these groups excepted blank group were added RAGE inhibitors FPS-ZM1, NF-κB inhibitor PDTC and mTOR inhibitor rapamycin (RAP) respectively, and the remaining three groups were treated with RAGE inhibitor FPS-ZM1, NF-κB inhibitor PDTC and mTOR inhibitor rapamycin (RAP) then added 50mg/mL concentrated Ziyin Huoxue Jiedu decoction to test. The expression of RAGE and mTOR was detected by Western-blot, and NF-κB nuclear translocation was detected by immunohistochemistry. Results The cells added with AGEs show abnormal growth, such as metamorphosis and shrink cells, membrane with hazy outline, roughness particles, which increase obviously. with increased cells. Inhibitor group and inhibitor added with Chinese medicine group could reverse this lesion with different degree. Inhibitor added with Chinese medicine group showed manny fusiform normal cells and few round and shrink cells. The expression of RAGE in inhibitor added with Chinese medicine group showed an obvious increase than inhibitor group (P<0.01). The expres-sion of mTOR in inhibitor added with Chinese medicine group showed a significant increase than inhibitor group (P<0.01). The positive detection rate of NF-κB drug-containing inhibitor was significantly lower than that of inhibitor group (P<0.01). Conclusion Ziyin Huoxue Jiedu decoction could reduce VEC damage induced by AGEs, and also could decrease the expres-sion of RAGE, NF-κB, mTOR.关键词
滋阴活血解毒方/AGEs/内皮细胞损伤/RAGE/mTOR/NF-κBKey words
Ziyin Huoxue Jiedu decoction/AGEs/endothelial cell injury/RAGE/mTOR/NF-κB分类
医药卫生引用本文复制引用
颜佳博,邓奕辉,廖俞强,李钰佳,李定祥..基于RAGE/NF-κB/mTOR信号通路探讨滋阴活血解毒方对AGEs诱导VEC损伤的干预作用[J].湖南中医药大学学报,2017,37(7):719-722,4.基金项目
湖南省教育厅项目(16K063) （16K063）
湖南省中医药科研基金(201606) （201606）
中西医结合基础湖南省重点学科资助. （）
