海洋科学2017，Vol.41Issue(3)：1-7,7.DOI:10.11759/hykx20160330001
兼养小球藻在不同浓度Fe3+培养下的蛋白质组学研究
Proteomic analysis of mixotrophic cultivation of Chlorella vulgaris exposed to different iron concentrations
摘要
Abstract
Here, we investigated the effect of different iron concentrations on the growth rate and lipid content of autotrophic and mixotrophic cultivations of Chlorella vulgaris. Differential expression of proteins in mixotrophic C. vulgaris exposed to different iron concentrations was analyzed. The results showed that mixotrophic cultivation demonstrated a faster growth rate and improved biomass than autotrophic cultivation. Iron deficiency may result in an increase in the lipid content. Proteomics analysis showed that the concentration of iron-containing proteins in-volved in photosynthesis was low in an iron-deficient culture condition. Hot shock proteins as well as proteins in-volved in translation and glycolysis were downregulated under both low and high iron concentrations, indicating that these growth conditions were stressful for C. vulgaris. The concentration of proteins involved in amino acid metabolism increased under iron-deficient conditions, possibly due to downregulation of NO3? assimilation and amino acid synthesis. These results showed that both the growth rate and lipid content in mixotrophic C. vulgaris were higher under iron-deficient conditions. The growth of C. vulgaris was accelerated under conditions of high iron concentrations, without affecting its lipid content. These results imply that high iron concentrations lead to a stress effect on the cells of C. vulgaris.关键词
小球藻(Chlorellavulgaris)/兼养/乙酸盐/铁离子/蛋白质组Key words
Chlorella vulgaris/mixotrophic/acetate/Fe3+/proteomics分类
农业科技引用本文复制引用
刘彩霞,顾文辉,黄爱优,伍松翠,张宝玉,王广策..兼养小球藻在不同浓度Fe3+培养下的蛋白质组学研究[J].海洋科学,2017,41(3):1-7,7.基金项目
科技部国际合作专项(2015DFG32160) （2015DFG32160）
科技部国家科技基础 性 工 作 专 项 (2012FY112900-01) （2012FY112900-01）
南 通 市 2014 年 科 技 计 划 项 目(AS2014007) （AS2014007）
山东省博士后创新计划项目(2014) [Foundation: International S & T Cooperation Program of China (2015DFG32160), Ministry of Science and Technology of PRC funda-mental research work (2012FY112900-01) （2014）
Nantong Science and Tech-nology Planning Project 2014 (AS2014007) （AS2014007）
Postdoctoral Innovation Project Special Funds of Shandong Province (2014)] （2014）
