海洋科学2017，Vol.41Issue(3)：8-16,9.DOI:10.11759/hykx20160121002
瘤背石磺表皮生长因子基因的克隆、结构及进化分析
Molecular cloning and characterization of EGF-like gene in Onchidium struma
摘要
Abstract
The complete cDNA sequence of the EGF-like gene was cloned from Onchidium struma for the first time. cDNA of the EGF-like gene was named Os-egf1; it consisted of 1158 bp containing a 846-bp ORF that encoded a peptide of 281 amino acids. Multiple alignment and conserved domain analysis indicated that this peptide chain contained two conserved EGF domains and one EGF-like domain. Each domain contained at least six cysteine residues (CX7 CX4–5 CX10–13 CXCX8 C) that can form three disulfide bonds between C1 and C3, C2 and C4, and C5 and C6. These characteristics are consistent with the EGF protein family. However, the remaining sequences of Os-egf1 were considerably different from other members of the EGF family. Thus, we proposed that Os-egf1 is a novel EGF-like gene. A phylogenetic tree was built using MEGA6.0 to investigate the relationship between Os-egf1 and proteins belonging to the EGF family. The study also suggested that proteins belonging to the EGF family are species-specific.关键词
瘤背石磺(Onchidiumstruma)/EGF/基因克隆/系统进化分析Key words
Onchidium struma/EGF/gene cloning/phylogenetic analysis分类
农业科技引用本文复制引用
杨铁柱,沈和定,史艳梅,刘欣,吴欣,李杰..瘤背石磺表皮生长因子基因的克隆、结构及进化分析[J].海洋科学,2017,41(3):8-16,9.基金项目
国家自然科学基金资助项目(41276157) （41276157）
上海高校水产学一流学科建设项目[Foundation: National Natural Science Foundation of China, No. 41276157 （）
Shanghai Fisheries College discipline construction project] （）
