海洋科学2017，Vol.41Issue(3)：41-47,7.DOI:10.11759//hykx20160314001
斑节对虾4个地理群体遗传多样性的AFLP分析
Genetic diversity in four populations of Penaeus monodon as-sessed by AFLP makers
摘要
Abstract
The genetic diversity of four Penaeus monodon populations (Africa population, F; Sanya population, S;Thailand population, T; Indonesia population, Y) was analyzed by the AFLP technique. One thousand bands were detected with eight primer combinations. The polymorphic bands of four populations and the ratio of polymorphic loci were 478–585 and 47.8%–58.5%, respectively. Nei's gene diversity index and Shannon's information index were 0.0917–0.1271 and 0.1484–0.2032, respectively. The genetic distance between populations F and Y was the largest (0.331), while it was the smallest between populations T and S (0.217). UPGMA dendrogram analysis showed that populations S, T and Y clustered onto one branch, and population F formed another branch. Except for four individuals, the other samples from population F clustered onto one branch, and most samples of populations S, T and Y clustered with their respective populations, while a small number of them were mixed together. AMOVA analysis showed that 35.92% of the variation was among populations and 64.08% variation was within populations. Fst (0.3592) among populations indicated large genetic differentiation. The germ styles of populations S, T and Y were similar, but they were different from that of population F. The results of this study accumulated information on the genetic background of Penaeus monodon that will be useful in genetics and breeding and can provide basic in-formation for further development of specific molecular markers in different populations.关键词
斑节对虾/遗传多样性/AFLP/地理群体Key words
Penaeus monodon/genetic diversity/AFLP/geographical populations分类
农业科技引用本文复制引用
孙苗苗,杨其彬,陈百尧,朱彩艳,黄建华,江世贵,周发林..斑节对虾4个地理群体遗传多样性的AFLP分析[J].海洋科学,2017,41(3):41-47,7.基金项目
现代农业产业技术体系(CARS47) （CARS47）
广东省海洋渔业科技与产 业 发 展 专 项 重 点 项 目 (A201501A06) （A201501A06）
广 东 省 科 技 计 划 项 目(2013B020201001) （2013B020201001）
广东省科技计划项目(2014B020202003) [Foundation: China Agriculture Research System, No.CARS-47 （2014B020202003）
Guang-dong Marine Fishery Science & Technology and Industry Development Special Key Project, No. A201501A06 （）
Science and Technology Planning Project of Guangdong Province, No.2013B020201001 （）
Science and Tech-nology Planning Project of Guangdong Province, No. 2014B020202003] （）
