海洋科学2017，Vol.41Issue(3)：48-54,7.DOI:10.11759//hykx20160422005
8种石首鱼类线粒体基因组特征及分子系统进化分析
Analysis of the mitochondrial genome characteristics and phylogenetic relationships of eight sciaenid fishes
摘要
Abstract
To explore the phylogenetic relationships of sciaenid fishes, the basic characteristics of mitochondrial genomes in eight sciaenid fishes (Collichthys niveatus, C. lucidus, Larimichthys crocea, L. polyactis, Miichthys miiuy, Pennahia argentata, Nibea albiflora, and Johnius belangerii) were revealed by comprehensive bioinformatic analysis. The results showed that mitochondrial genomes contained 37 standard vertebrate genes, and the order was identical except for in J. belangerii. The base distribution was not balanced, and the content of A+T was higher than that of G+C. Genetic variation analysis of 13 protein-coding genes and 2 ribosomal RNA genes among sciaenid fishes showed that the ND4 and ND5 genes could be used as supplementary molecular markers to the COI gene and applied to research on population genetic diversity. The Ka/Ks ratios of 13 mitochondrial protein-coding genes in five Pseudosciaeninae fishes were much lower than 1, indicating strong purifying selection. The genetic distances between J. belangerii and other sciaenid fishes were high, and relationships were distant, which suggested that Johnius fishes might be the relatively primitive group. A neighbor-joining phylogenetic tree based on the complete mitochondrial genome supported the morphological conclusion that fishes of Pseudosciaeninae and Argyrosominae were closely related. Furthermore, phylogenetic trees based on sequences deleting the control region and 13 pro-tein-coding genes showed that the differences between these two subfamilies were much more evident.关键词
石首鱼类/线粒体基因组序列/系统进化Key words
Sciaenid fishes/mitochondrial genome sequence/phylogenetic分类
农业科技引用本文复制引用
孙利元,杨天燕,孟玮,杨宝清,张涛..8种石首鱼类线粒体基因组特征及分子系统进化分析[J].海洋科学,2017,41(3):48-54,7.基金项目
山东省海洋与渔业厅资助项目(No. 270006-FZLX-2015-00-1) [Foundation: Department of Ocean and Fisheries of Shandong Province Program, No. 270006-FZLX-2015-00-1] （No. 270006-FZLX-2015-00-1）
