渔业科学进展2017，Vol.38Issue(3)：124-132,9.DOI:10.11758/yykxjz.20160304002
聚β-羟基丁酸酯对中国明对虾(Fenneropenaeus chinensis)非特异性免疫相关酶的影响
The Effect of Poly-β-Hydroxybutyrate on Enzyme Activities of Nonspecific Immunity in Fenneropenaeus chinensis
摘要
Abstract
In the present study,we explored the effect of poly-β-hydroxybutyrate (PHB) on the performance of the immune system in Fenneropenaeus chinensis.This study used single factor concentration gradient method.F.chinensis were fed with baits containing PHB at different concentrations (0,0.5％,1.0％,2.5％ and 5.0％).After six weeks,the mortality and relative immune protective rate of each group was calculated.Moreover,enzyme activities of nonspecific immunity,including the total antioxidant capacity (T-AOC),the enzyme activities of acid phosphatase (ACP),peroxidase (POD) and catalase (CAT),and the content of malondialdehyde (MDA) in muscle,hepatopancreas and serum were analyzed and compared between groups on a weekly basis.The results showed that the PHB had a significant effect on immune performance.With the increase of PHB concentration,the relative immune protective rates in the experimental groups were elevated initially followed by a decline.The group of E1.0 had the highest relative immune protective rate compared with other groups (P＜0.05).The trend of the enzyme activities were similar to the changes of the relative immune protective rates.On the spatial distribution,the enzyme activities of T-AOC,POD,CAT in serum and ACP in hepatopancreas were the highest.On the temporary distribution,the total enzyme activities reached the highest level after 2-3 weeks of treatments.Among different groups,the groups of E1.0 and E2.5 in serum and E1.0 in hepatopancreas had significant different T-AOC from other groups (P＜0.05).The ACP activities in serum in the groups of E1.0 and E2.5,and E1.0 in the hepatopancreas were significantly different from other groups (P＜0.05).During the high level expression period of CAT,serum CAT in E0.5,E1.0,E2.5 groups,hepatopancreas CAT in E 0.5,E1.0,E10.0 groups were significantly different from other groups (P＜0.05).The serum and hepatopancreas POD activities in E0.5,E1.0,E2.5 groups were significantly different from other groups (P＜0.05).The SOD activities in serum and hepatopancreas in group E1.0 were significantly different from other groups (P＜0.05).And the content of MDA in serum in E1.0 group and that in hepatopancreas in group E0.5 and E1.0 were significantly different from other groups.Based on the results,it is concluded that the PHB can improve the immunity ofF.chinensis.Furthermore,1.0％ PHB inclusion in feed had the best effect and the total enzyme activities reached the highest level after 2-3 weeks of treatment.关键词
中国明对虾/聚β-羟基丁酸酯/相对免疫保护率/酶活力Key words
Fenneropenaeus chinensis/Poly-β-hydroxybutyrate (PHB)/Relative immune protective rates/Enzyme activities分类
农业科技引用本文复制引用
张恒恒,孟宪红,孔杰,罗坤,栾生,曹宝祥,曹家旺,张莹雪..聚β-羟基丁酸酯对中国明对虾(Fenneropenaeus chinensis)非特异性免疫相关酶的影响[J].渔业科学进展,2017,38(3):124-132,9.基金项目
中国水产科学研究院基本科研业务费(No.2016GH06)、国家自然科学基金(31372523)、青岛海洋科学与技术国家实验室鳌山科技创新计划项目(No.2015ASKJ01)和泰山学者良种工程项目共同资助[This work was supported by Central Public-Interest Scientific Institution Basal Research Fund,CAFS(No.2016GH06) （No.2016GH06）
National Natural Science Foundation of China (31372523) （31372523）
The Scientific and Technological Innovation Project Financially Supported by Qingdao National Laboratory for Marine Science and Technology (No.2015ASKJ01),and Taishan Scholar Program for Seed Industry]. （No.2015ASKJ01）
