渔业科学进展2017，Vol.38Issue(3)：180-187,8.DOI:10.11758/yykxjz.20160505001
刺参(Apostichopus japonicus)大水面养殖池塘环境中优势益生菌筛选及其特性分析
Screening and Characteristic Analysis of Potential Probiotics from Large Water Ponds Used for Sea Cucumber (Apostichopus japonicus) Farming
摘要
Abstract
From December 2014 to December 2015,the flora population structural analysis and beneficial bacteria isolation and screening were carried out in large water ponds of a sea cucumber (Apostichopus japonicus) farm.Sixty-six bacterial strains were isolated from the water body and pond sediment.Two bacterial strains Pseudoalteromonas nigrifaciens and Vibrio splendidus associated with the "skin ulcerative syndrome" disease were used as indicators in the antagonistic effect trials.Based on the bacterial extracellular amylase,protease activities and challenge tests,one potential probiotic bacterium YQ-2 was obtained.The results showed that this strain had good ability to inhibit the proliferation of P nigrifaciens and V.splendidus,evident by the diameter of inhibition zones,22 mm and 24 mm,respectively;while the diameter of amylase and protease hydrolysis was 22 mm and 36 mm;In addition,the challenge tests revealed that the strain YQ-2 was safe to sea cucumber when it was challenged by bathing the bacterial strain at the concentration of 108 CFU/ml,or fed the food inoculated with the bacterial cells at the concentration of 108 CFU/g once daily for one month.The present study also found that among the sea cucumbers fed the food containing 108 CFU/g bacterial cells,the body weight increased significantly compared with the control group,with the relative growth rate as high as 39.31％.Homogenous analysis using 16S rDNA sequences indicated that the strain YQ-2 had 99％ similarity with the Bacillus subtilis strain KLP2015.Thus,the strain YQ-2 was tentatively identified as Bacillus subtilis.The number of B.subtilis in the rearing water was counted in all four seasons and the year-round concentration was about 140-280 CFU/ml,which was higher than other strains.Meanwhile the dominance of YQ-2 was 4.2％,3.5％,2.6％,and 4.6％ corresponding to Spring,Summer,Autumn and Winter.Furthermore,the dominance of YQ-2 was higher in Spring and Winter compared to Summer and Autumn.Nevertheless,the strain YQ-2 exhibited a strong antibacterial effect on the pathogens P.nigrifaciens and V.splendidus which were the main pathogenic agents causing the "skin ulcer syndrome" disease.Hence,the strain YQ-2 has great application for farming A.japonicus in large water ponds.关键词
刺参/大水面池塘/腐皮综合征/益生菌/枯草芽孢杆菌/拮抗菌Key words
Apostichopus japonicus/Large water pond/Skin ulcerative syndrome/Probiotic bacterium/Bacillus subtilis/Antagonistic bacteria分类
农业科技引用本文复制引用
杜佗,李彬,王印庚,廖梅杰,王金燕,刘志轩,王诗欢..刺参(Apostichopus japonicus)大水面养殖池塘环境中优势益生菌筛选及其特性分析[J].渔业科学进展,2017,38(3):180-187,8.基金项目
中央级科研院所基本科研业务费专项资金项目(20603022015015)、山东省自主创新成果转化专项(2013ZHZX2A0801)和青岛市海洋经济创新发展区域示范项目(海水健康养殖专用微生态制剂的开发应用与产业化项目)共同资助[This work was supported by Special Fund for Basic Scientific Research Business of Central Public Research Institutes (20603022015015),Independent Innovation and Achievements Transformation of Shandong Province (2013ZHZX2A0801),and Regional Demonstration Project of Marine Economy Innovation and Development in Qingdao City (Development and Application of Micro Ecological Agent Special for Sea Water Health Cultivation and Industrialization Project)]. （20603022015015）
