家畜生态学报2017，Vol.38Issue(6)：8-12,5.
测定猪不同组织基因表达时RT-PCR内参基因的选择
Selection of Reference Genes of Real-time Quantitative PCR in the GeneExpression of Different Tissues in Pig
摘要
Abstract
Taking different organizations of 95 kg weight fatten pigs,the expressions of 11 housekeeping genes (SDHA),(HMBS),(TOP2B),(ACTB),(B2M),(PPIA),(GAPDH),(HPRT1),(RPL4),(TBP) and (YWHAZ) were detected using real-time quantitative PCR.And the stability of internal genes was evaluated by geNorm program.Results of geNorm program analysis showed that TBP and RPL4 were stable expression internal genes in muscle tissue, GAPDH and RPL4 were stable expression internal genes in heart tissue, TBP and HPRT1 were stable expression internal genes in liver tissue, SDHA and ACTB were stable expression internal genes in spleen tissue, B2M and HPRT1 were stable expression internal genes in lung tissue.Thus it can be seen that the most stable expression internal genes in different tissues of pig are different,and the internal genes to standardize target genes in different tissues are also different.关键词
猪/实时荧光定量PCR/内参基因选择/geNorm程序/基因表达Key words
pig/real-time quantitative PCR/selection of the reference genes/GeNorm program/gene expression分类
农业科技引用本文复制引用
齐波,朱荣生,王怀中,孙守礼,王建才,刘俊珍,黄保华,呼红梅..测定猪不同组织基因表达时RT-PCR内参基因的选择[J].家畜生态学报,2017,38(6):8-12,5.基金项目
国家自然科学基金(31501928) （31501928）
山东省科技发展计划(2015GNC111011) （2015GNC111011）
山东省农业科学院青年基金(2014QNM41) （2014QNM41）
山东省自然基金(ZR2015CM007) （ZR2015CM007）
山东省农业科学院科技创新重点项目(2014CX202) （2014CX202）
