激光生物学报2017，Vol.26Issue(2)：162-167,6.DOI:10.3969/j.issn.1007-7146.2017.02.012
贵州侗族人群MICB等位基因多态性研究
Study on the Allele Polymorphism of MICB in Dong Nationality of Guizhou
摘要
Abstract
Objective: To study the characterization of MICB alleles in a healthy Dong nationality of Guizhou.Methods: Fresh blood samples were collected from one hundred health Dong population in Guizhou.Genomic DNA was extracted from the peripheral blood using the standard salt precipitation method recommended by World Health Organization.MICB genotypes were determined by using PCR-sequence-specific primers (PCR-SSP) and PCR-sequence-based typing (PCR-SBT),respectively.Results: Seven known MICB alleles in total were observed in the tested subjects.Among the characterized MICB alleles,MICB*005:02 showed the highest allele frequency (58.50%),followed by the allele frequency of MICB*002:01 (22.00%).Furthermore,the allele frequency of MICB*003 and MICB*005:03 were the lowest in the tested individuals (1.50%).Conclusions: MICB allele showed high polymorphism in Dong nationality of Guizhou.These results could serve as the basis for future studies on the potential role of MICB in allogeneic organ transplantation and disease susceptibility in related ethnic groups.关键词
基因多态性/MICB基因/贵州侗族Key words
gene polymorphism/MICB alleles/Dong nationality of Guizhou分类
医药卫生引用本文复制引用
李沈明,陈富超,陈纯静,余平..贵州侗族人群MICB等位基因多态性研究[J].激光生物学报,2017,26(2):162-167,6.基金项目
国家自然科学基金(81572040) （81572040）
