激光生物学报2017，Vol.26Issue(2)：189-192,封3,5.DOI:10.3969/j.issn.1007-7146.2017.02.016
转录因子AP-2beta和p53蛋白相互作用的研究
A Study on the Interaction of Transcription Factor AP-2beta and p53 Protein
摘要
Abstract
The mutation of transcription factor p53 and AP-2beta which is AP-2 gene family member will result in individual diseases.Firstly,the use of two recombinant plasmids pCMV-HA-p53 and pMyc-AP-2beta to transfect immortalized embryonic kidney cells HEK293 (wild-type).Co-immunoprecipitation experiments showed that AP-2beta and p53 proteins may interact in vivo,And then the gradient of increasing pMyc-AP-2beta and the same amount of plasmid pCMV-HA-p53 were transfected into HEK293 cells.The results showed that AP-2beta can regulates of p53 positively by Western-blot.To further investigate the mechanism of interation of AP-2beta and p53,cells which were co-transfected AP-2beta and p53 plasmid were incubated with cycloheximide (CHX),experiments showed that AP-2beta can increased the stability of p53 proteins.关键词
AP-2beta/p53/蛋白稳定性Key words
AP-2beta/p53/the stability of protein分类
生物科学引用本文复制引用
王琳,汤婷,欧阳文英,粟敏..转录因子AP-2beta和p53蛋白相互作用的研究[J].激光生物学报,2017,26(2):189-192,封3,5.基金项目
湖南省教育厅一般项目([2013]13C1118) （[2013]13C1118）
