激光生物学报2017，Vol.26Issue(3)：217-223,7.DOI:10.3969/j.issn.1007-7146.2017.03.005
ITO石英晶体电极上不同内皮细胞浓度的粘弹性研究及药物作用评估
Real Time Monitoring of Viscoelasticity of Different Human Umbilical Vein Endothelial Cell Concentrations on ITO Quartz Crystal Electrode and Evaluation of Cells-Drug Interactions Using Quartz Crystal Microbalance
摘要
Abstract
In this study,the dynamic response of human umbilical vein endothelial cells (HUVECs) to ITO quartz crystal electrodes was monitored by quartz crystal microbalance (QCM) and live cell imaging.HUVECs with different cell concentrations were added to the ITO electrode.Real-time changes of QCM resonance frequency and dissipation were measured and compared with different concentrations of HUVEC.Through the combination of ITO electrode and optical microscope,the dynamic changes of HUVEC before and after drug treatment were also monitored.The Cell Viscoelastic Index (QCM ratio of dynamic resistance change to frequency shift,CVI=ΔR/ΔF) was used to characterize the cells'' viscoelasticity,at the same time the changes of cell morphological were real-time monitored by living cell imaging technology.The results showed that when the cell concentration was 10 000 cells/mL,the CVI was the maximum on the ITO electrode and HUVEC spread completely.The morphological changes of the cells were not obvious under the real-time monitoring of the microscope before and after drug treatment,but the changes of CVI was great.The results showed that the QCM signal was more sensitive than the optical signal.关键词
晶体电极/内皮细胞/石英晶体微天平(QCM)/活细胞成像技术Key words
ITO electrode/endothelial cells/quartz crystal microbalance (QCM)/living cell imaging分类
信息技术与安全科学引用本文复制引用
鲍冬芹,潘炜松,谭成方,沈海波,周铁安..ITO石英晶体电极上不同内皮细胞浓度的粘弹性研究及药物作用评估[J].激光生物学报,2017,26(3):217-223,7.基金项目
国家自然科学基金面上项目(21275048) （21275048）
湖南省科技厅重点项目 (2013TT1009) （2013TT1009）
