激光生物学报2017，Vol.26Issue(3)：260-265,6.DOI:10.3969/j.issn.1007-7146.2017.03.012
不同方法检测鸡蛋表面菌落总数的相关性研究
A Study on the Correlation of Different Methods for Detecting the Total Number of Bacteria on the Surface of Eggs
摘要
Abstract
In this study,the bacterial count of the egg shell surface was compared by two methods: GB4789.2-2010 microbiological examination of food Hygiene: detection of aerobic bacterial count,ATP bioluminescence assay to study the correlation between ATP count and routine bacterial detection.The total bacteria count on the surface of 40 mixed samples was detected by ATP fluorescence detection,and the national standard method was used as the control.The Pearson correlation coefficient of ATP fluorescence test and the result of national standard method was 0.912.On the basis of these results,with log (CFU) as abscissa (x) and log (RLU) as ordinate (y),the linear fitting,logarithm fitting and exponent fitting were conducted.The fitting degree logarithmic model y=0.7306x-1.0041(R2=0.8322) was higher.ATP fluorescence detection method and the national standard method in the egg shell surface bacterial load detection results have a good correlation.ATP fluorescence detection can be replaced to some extent,routine testing of bacteria and applied to the actual production.关键词
蛋壳/ATP生物荧光法/细菌菌落总数/相关性Key words
eggshell/ATP bioluminescence method/the total number of bacterial colonies/分类
轻工纺织引用本文复制引用
程教擘,李梦丹,刘金,谢艳华,陈力力,刘焱..不同方法检测鸡蛋表面菌落总数的相关性研究[J].激光生物学报,2017,26(3):260-265,6.基金项目
公益性行业(农业)科研专项(201303084) （农业）
湖南省自然科学基金(61275118) （61275118）
