激光生物学报2017，Vol.26Issue(3)：266-273,8.DOI:10.3969/j.issn.1007-7146.2017.03.013
三叶木通促分裂原活化蛋白激酶基因mapk3的克隆及表达分析
Cloning and Expression Analysis of Mitogen-Activated Protein Kinases Gene of mapk3 of Akebia trifoliata
摘要
Abstract
Plant MAPK (mitogen-activated protein kinases) cascades play a crucial role in the process of hormone signal transduction,various stresses responses and plant growth and development.The full-length cDNA sequence of Akebia trifoliata mapk3 gene was amplified by using the technology of rapid amplification of cDNA ends (RACE) method.Subsequently,characterization of Aktmapk3,including gene alignment,phylogenetic tree,conserved domain and protein structure were analyzed by bioinformatics,while the expression level of mapk3 in different tissues of Akebia trifoliata were analyzed by real-time quantitative PCR method.Result showed that the Aktmapk3 gene contained an 1 164 bp open reading frame (ORF) which encodes 387 amino acids,and the coding protein contained an ATP binding site (IPR017441),MAPK conserved site (IPR003527),and Serine/threonine-protein kinase active site (IPR008271) suggesting that this protein might be activated by phosphorylation,and then phosphorylate downstream protein to executive physiologic function.The result of Real-time PCR exhibited that Aktmapk3 was the highest abundance in bud,mature leaf and flower,suggested that Aktmapk3 might be involved in the development of bud and flower.关键词
三叶木通/促分裂原活化蛋白激酶/克隆/基因表达Key words
Akebia trifoliata/MAPK3/cloning/gene expression分类
生物科学引用本文复制引用
吴小祝,文锋,廖亮,罗海霞,李鹏..三叶木通促分裂原活化蛋白激酶基因mapk3的克隆及表达分析[J].激光生物学报,2017,26(3):266-273,8.基金项目
国家自然科学基金(31560075) （31560075）
江西省青年科学基金(20151BAB214003) （20151BAB214003）
江西省卫生计生委中药研究项目(2015A075) （2015A075）
