江西农业大学学报2017，Vol.39Issue(3)：559-566,8.DOI:10.13836/j.jjau.2017072
一株海洋放线菌抗菌活性物质的分离与结构解析
Isolation and Structure Identification of the Antimicrobial Compounds from a Marine Actinomycetes Strains
摘要
Abstract
To discover the new resource of antimicrobial activity compound from marine actinomycetes,a marine actinomycetes Streptomyces tendae was screened out with significant antibacterial activity to Bacillus subtilis and Staphylococcus aureus from marine actinomycetes strain library.Then the fermentation conditions for the antibacterial marine actinomycete were optimized,and the strongest antibacterial activity of the fermentation liquor was observed when the strain was cultured with 2216E medium for 96 h,under 28 ℃ and shaking under 180 rpm.Furthermore the antibacterial activity compounds from the antibacterial marine actinomycete were isolated and purified based on activity tracking and combined with chromatographic separation techniques.And the structure of the isolated compound was identified by means of NMR and high resolution mass spectrometry.The results showed that the two antimicrobial compounds from the antibacterial marine actinomycete were identified as 2-Hydroxy-3-propyl-hexahydro-pyrrolo[1,2-a] pyrazine-1,4-dione and 3-Propyl-hexahydro-pyrrolo[1,2-a] pyrazine-1,4-dione,and they were derivatives of cyclic dipeptides.关键词
海洋放线菌/抗菌活性/环二肽/天然产物/唐德链霉菌Key words
marine actinomycetes/antimicrobial activity/cyclic dipeptides/nature product/Streptomyces tendae分类
生物科学引用本文复制引用
林真亭,叶子坚,庄玲萍,许莉,张岗,强华..一株海洋放线菌抗菌活性物质的分离与结构解析[J].江西农业大学学报,2017,39(3):559-566,8.基金项目
福建省教育厅自然科学基金项目(JA13424)和厦门市海洋与渔业局产业技术攻关项目(14GYY74NF38) Project supported by the Natural Science Foundation of Fujian Provincial Department of Education(JA13424)and Industrial Technology Research Project of Xiamen Oceanic and Fishery Bureau(14GYY74NF38) （JA13424）
