江西农业大学学报2017，Vol.39Issue(3)：567-571,5.DOI:10.13836/j.jjau.2017073
五谷虫抑菌活性多肽RP-HPLC分离与纯化方法研究
A Study on Separation and Purification of Antibacterial Peptides in Maggot with RP-HPLC
摘要
Abstract
This study aims at establishing a method for separation of antibacterial peptides from Maggot and providing experimental evidence for further study on its medicinal value.Peptides,with molecular weight below 30ku were obtained through the process of homogenization,filtrating,heating,centrifuging and ultrafiltration.Subsequently,the method of SuperdexTM30 gel filtration chromatography was utilized to separate peptides and to identified the antibacterial activity peaks,which were further purified with RP-HPLC.The antibacterial components of S2 and S3 were screened out with gel filtration chromatography.Besides,seven peaks in S2 and four peaks in S3 were identified with RP-HPLC.The RSD of retention time and peak area were all less than 5% in stability,precision and repetition experiment,and the similarity of 6 bathes of samples was more than 0.99.The method of RP-HPLC can be used to quickly separate antibacterial polypeptides from Maggot,and it is stable,reliable and reproducible.关键词
五谷虫/抑菌肽/RP-HPLC/分离/纯化Key words
Maggot/antibacterial peptides/RP-HPLC/separation分类
医药卫生引用本文复制引用
王敏,褚夫江,巫春旭,刘文彬,金小宝,朱家勇..五谷虫抑菌活性多肽RP-HPLC分离与纯化方法研究[J].江西农业大学学报,2017,39(3):567-571,5.基金项目
国家自然科学基金项目(81274061) Project supported by the National Natural Science Foundation of China(81274061) （81274061）
