临床口腔医学杂志2017，Vol.33Issue(6)：326-330,5.DOI:10.3969/j.issn.1003-1634.2017.06.003
不同蔗糖浓度下外源性右旋糖酐酶协同氟化钠对S.Mutans生物膜形成的影响
Effects of exogenous dextranase and sodium fluoride on biofilm formation of Streptococcus mutans in different sucrose concentration medium
摘要
Abstract
Objective:To investigate the effects of exogenous dextranase(Dex)and sodium fluoride(NaF)in different sucrose concentration medium on biofilm formation of Streptococcus mutans(S.Mutans).Methods:The model of S.Mutans biofilmin in vitro was established.The pH value of the medium with different sucrose concentration during the biofilm formation was detected by pH electrode.Crystal violet staining was used to observe the effects of Dex and NaF on biofilm formation.Scanning electron microscope was applied to detect the inhibitory effect of Dex and NaF on biofilm formation.Results:In different sucrose concentration medium,Dex had no effect on the pH value,but NaF could increase the pH value.The pH values of the Dex+NaF groups were higher than the control groups(P<0.05),and significant difference was existed among Dex+NaF groups with different sucrose concentration(P<0.05) except the 3% and 4% groups.The mass of biofilm in all treated groups decreased when compared with the control groups,and Dex and NaF had synergistic inhibitory effect on the biofilm formation.There were statistically significant differences in biofilm formation between Dex+NaF groups and control groups(P<0.05).When simultaneously treated with Dex and NaF,the formation of biofilm decreased when the sucrose concentration increased.Conclusion:Dex and NaF had inhibitory effect on the biofilm formation of S.Mutans,and they had synergistic effect when used together.Sucrose concentration could also affect the biofilm formation of S.Mutans.关键词
变异链球菌/生物膜/右旋糖苷酶/氟化钠/扫描电子显微镜Key words
Streptococcus mutans/Biofilm/Dextranase/NaF/Scanning electron microscopy(SEM)分类
医药卫生引用本文复制引用
喻譞,田媛媛,杨英明,房宏志,胡涛,杨惠..不同蔗糖浓度下外源性右旋糖酐酶协同氟化钠对S.Mutans生物膜形成的影响[J].临床口腔医学杂志,2017,33(6):326-330,5.基金项目
国家自然科学基金(81400507) （81400507）
四川省科技厅应用基础研究项目(2013JY0164) （2013JY0164）
