临床误诊误治2017，Vol.30Issue(6)：103-107,5.DOI:10.3969/j.issn.1002-3429.2017.06.036
骨髓来源细胞表达的MMP-2/MMP-13在脉络膜新生血管发生发展中的作用
Effect of MMP-2/MMP-13 Expression by Bone Marrow-derived Cells in Development of Choroidal Neovascularization
摘要
Abstract
Objective To investigate matrix metalloproteinase (MMP) dynamic expression by bone marrow-derived cells (BMCs) and its relationship with pathogenesy and development of choroidal neovascularization (CNV).Methods A total of 24 C57BL/6J green fluorescent protein (GFP) of allophenic mice, who underwent bone marrow transplantation successfully, were selected as observation group, while other 48 C57BL/6J mice without bone marrow transplantation were selected as control group.CNV was induced by irradiating with laser photocoagulation for all mice.In control group, MMP-2/MMP-13 expressions were detected by Western blot and gelatin zymography method before induction, and at 1st, 3rd, 5th, 7th, 10th, 14th and 28thd after induction;In observation group, immunofluorescence staining was used to identify whether or not MMP-2/MMP-13 was expressed by GFP-positive BMCs in CNV, and quantitative analysis was also performed.Results Western blot, gelatin zymography and immunofluorescence staining methods all showed that MMP-2 expressions were rapidly increased in the early laser stage of CNV patients in two groups, and the expressions of BMCs and MMP-2 reached the highest values and strongest activity at 3rd d after induction;the highest value of MMP-2 expression in observation group occupied 63.21% of the total expression;and then MMP-2 expression began to decline.MMP-13 expression showed slowly increases at the early laser stage of CNV patients in two groups, and the highest expression and strongest activity were found at 7th d after induction;the highest values of MMP-13 expression in CNV patients in two groups and in observation group occupied 77.87% of the total expression;and then MMP-13 expression began to decline.Conclusion BMCs can be used as target point to regulate MMP-2/MMP-13 expression, and it can further affect pullulation, transmigration, apoptosis and fibration of CNV.关键词
脉络膜新生血管化/骨髓来源细胞/基质金属蛋白酶Key words
Choroidal neovascularization/Bone marrow derived cells/Matrix metalloproteinase分类
医药卫生引用本文复制引用
吕洋,李红,岳红云,姚长江..骨髓来源细胞表达的MMP-2/MMP-13在脉络膜新生血管发生发展中的作用[J].临床误诊误治,2017,30(6):103-107,5.基金项目
国家自然科学基金资助项目(81500748) （81500748）
甘肃省自然基金项目(1308RJZA249) （1308RJZA249）
