临床与实验病理学杂志2017，Vol.33Issue(5)：501-504,4.DOI:10.13315/j.cnki.cjcep.2017.05.007
沉默缺氧诱导因子-2α对乳腺癌细胞化疗敏感性的影响
Effect of silencing of HIF-2α gene on chemosensitivity of human breast carcinoma cells
摘要
Abstract
Purpose To investigate the effects of hypoxia inducible factor-2α (HIF-2ot) siRNA on proliferation and chemotherapy sensitivity of breast carcinoma MCF-7.Methods RNA interference was used to silence the expression of HIF-2α in MCF-7 cells.The changes of HIF-2α gene expression were detected by immunocytochemistry and RT-PCR.Under hypoxia environment simulated by CoCl2,MTT assay and flow cytometry (FCM) were used to measure cell growth inhibition rate and cell apoptosis of MCF-7 cells under different dosages of chemotherapeutic agents (5-fluorouracil,adriamycin,and paclitaxel).Results Expression of HIF-2α in MCF-7 were down-regulated by HIF-2α siRNA (P ＜ 0.05).The proliferation inhibition and apoptosis rates were evidently increased after transfection with HIF-2α siRNA (P ＜ 0.05),chemotherapy drug sensitivity was enhanced.Conclusion HIF-2α siRNA can induce the apoptosis and inhibit the proliferation and enhance the sensitivity of breast carcinoma MCF-7 cell line to chemotherapeutic agents.Blocking HIF-2α maybe a very promising strategy for breast carcinoma gene therapy in combination with chemotherapy.关键词
乳腺肿瘤/缺氧诱导因子-2α/RNA干扰/化疗敏感性Key words
breast neoplasm/hypoxia inducible factor-2 alpha/RNA interference/chemosensitivity分类
医药卫生引用本文复制引用
李娜,贺国洋,李永真,王志慧..沉默缺氧诱导因子-2α对乳腺癌细胞化疗敏感性的影响[J].临床与实验病理学杂志,2017,33(5):501-504,4.基金项目
河南省自然科学基金面上项目(162300410220)、河南省卫生厅科技攻关资助(201403133)、河南省教育厅高等学校重点科研(16A310002) （162300410220）
