棉花学报2017，Vol.29Issue(3)：222-232,11.DOI:10.11963/1002-7807.cpyzj.20170322
陆地棉KFB基因家族全基因组鉴定与表达分析
Genome-Wide Identification and Expression Analysis of the KFB Gene Family in Gossypium hirsutum L.
摘要
Abstract
[Objective] The KFB (Kelch containing F-box protein) family,involving in many physiological pathways,is a subfamily of the biggest F-box protein family in all organisms.In the study,we aims to identify the KFB family members in the upland cotton genome and analyze their expression patterns.[Method] The KFB genes were identified from the whole genome of upland cotton (Gossypium hirsutum L.acc.TM-1) by bioinformatics methods.A phylogenetic analysis was conducted based on the alignment of the KFB proteins from upland cotton,Arabidopsis,rice and soybean,and the gcne structure,chromosomal localization,and expression of subgroup Ⅱ in this family.[Result] A total of 150 KFB genes were identified from the whole genome of upland cotton and were then divided into seven subgroups based on phylogenetic analyses.In subgroup Ⅱ,24 genes distributed on fourteen chromosomes,and nine pairs were orthologous genes.Structures of half genes in this subfamily were simple,including only one exon,respectively.According to the results of qRT-PCR,this subfamily had a wide range of tissue expression patterns,but the expression level of these genes varied from different tissues.[Conclusion] The results indicate that the functions of subgroup Ⅱ in KFB family have been differentiated during the process of evolution.关键词
陆地棉/KFB基因家族/生物信息学/基因表达Key words
Gossypium hirsutum/KFB gene family/bioinformatics/gene expression分类
农业科技引用本文复制引用
陈鹏云,张景霞,陈煜,刘国栋,张传云,王芙蓉,刘志,张军..陆地棉KFB基因家族全基因组鉴定与表达分析[J].棉花学报,2017,29(3):222-232,11.基金项目
国家科技重大专项(2014ZX0800501B-003) （2014ZX0800501B-003）
国家自然科学基金(U1403284) （U1403284）
山东自然科学基金(ZR2014CQ047) （ZR2014CQ047）
泰山学者建设工程专项(NO.ts201511070) （NO.ts201511070）
