南京医科大学学报（自然科学版）2017，Vol.37Issue(5)：554-558,5.DOI:10.7655/NYDXBNS20170507
二甲双胍对甲状腺未分化癌侵袭转移的影响及机制研究
The study on the effect of metformin on invasion and metastasis of undifferentiated thyroid cancer and its underlying mechanisms
摘要
Abstract
Objective:To observe the effects of mefformin on the invasion and metastasis of undifferentiated thyroid cancer and to evaluate its underlying mechanisms.Methods:The undifferentiated thyroid cancer cell line SW1736 was treated with different concentrations of mefformin.The invasive and metastatic abilities of SW1736 cells were measured by agar drop method and Transwell invasion and migration assay.Real-time PCR was used to detect the molecules mRNA involved in the process of epithelial-mesenchymal transition (EMT),including vimentin,E-cadherin and transcription factors Snail1 and Slug.Results:Metformin inhibited the invasive and migration capacities of SW1736 cells in vitro.The inhibitory effects of metformin were dose dependent.Mefformin significantly down-regulated the expressions of vimentin,Slug and Snail 1 in SW1736 cells.Meanwhile,it stimulated the expression of E-cadherin,which highly expresses in epithelial cells.Conclusion:Metformin can promote the expression of E-cadherin by down-regulating the expression of Snail1 and Slug,thus inhibiting the process of EMT and decreasing the invasive and migration ability of thyroid carcinoma,which may provide a novel strategy for the treatment of thyroid neoplasms.关键词
二甲双胍/甲状腺未分化癌/侵袭/转移/上皮间质转化Key words
metformin/undifferentiated thyroid cancer/invasion/metastasis/epithelial-mesenchymal transition分类
医药卫生引用本文复制引用
张丽娟,郏文亭,兰玲,钱玮,杨雪阳,徐宽枫,崔岱..二甲双胍对甲状腺未分化癌侵袭转移的影响及机制研究[J].南京医科大学学报（自然科学版）,2017,37(5):554-558,5.基金项目
江苏省“六大人才高峰”高层次人才项目(WSN-023) （WSN-023）
