南京医科大学学报（自然科学版）2017，Vol.37Issue(5)：584-587,624,5.DOI:10.7655/NYDXBNS20170513
阻断白介素-17对博来霉素诱导小鼠肺纤维化及肺组织Fas/FasL表达的影响
Blocking effects of interleukin-17 on pulmonary fibrosis and expression of Fas/FasL in mice induced by bleomycin
摘要
Abstract
Objective:To explore the effect on pulmonary fibrosis,cell apoptosis and expression of Fas/FasL in lung by blocking IL-17 activity in mice induced by bleomycin (BLM).Methods:Eighty C57BL/6 mice were randomly divided into the following 4 groups:sham group(SG),BLM group(BG),neutralizing antibody group(NG) and isotype-matched control antibody group(IG),respectively.Three groups were received a single intratracheal instillation of 5 mg/(kg body weight) of BLM to induce pulmonary fibrosis,while SGs was administrated the equvalent sterile saline.NG and IG were injected through caudal vein with neutralizing rat antimouse IL-17 mAb,or control rat IgG every 3 days starting on day 1 before making model,SG and BG were received equvalent PBS alone.All mice were sacrificed after 28 days.Lung tissues were removed and used to evaluate the extent of pulmonary fibrosis by Masson staining and hydroxyproline contents measurement.Cell apoptotic rate and the expression of Fas/FasL in mice were detected through Flow cytometry and immunohistochemical method.Results:Compared with BG and IG,the pulmonary fibrosis degree of NG was decreased remarkably(P＜0.01).Hydroxyproline contents was reduced obviously (P＜0.01).Apoptotic rate and expressions of Fas/FasL were decreased significantly (P＜0.01).Conclusion:Pulmonary fibrosis was improved significantly after the endogenous IL-17 activity blocked,meanwhile,apoptosis and the expressions of Fas/FasL were both decreased.These data showed that antimouse IL-17 mAb ameliorate the pulmonary fibrosis induced by BLM and it is related to Fas/FasL apoptotic pathways.关键词
白细胞介素-17/细胞凋亡/肺纤维化/Fas/FasLKey words
interleukin-17/apoptosis/pulmonary fibrosis/Fas/FasL分类
医药卫生引用本文复制引用
张效云,宋桂芹,辇晓峰,黄勇,郝敏,孙冲..阻断白介素-17对博来霉素诱导小鼠肺纤维化及肺组织Fas/FasL表达的影响[J].南京医科大学学报（自然科学版）,2017,37(5):584-587,624,5.基金项目
张家口市科技局自然科学基金资助(12110046D-1) （12110046D-1）
河北省高等学校自然科学重点项目(ZD2017206) （ZD2017206）
