日用化学工业2017，Vol.47Issue(6)：312-316,340,6.DOI:10.13218/j.cnki.csdc.2017.06.003
茶皂素的纯化及抑制酪氨酸酶活性的研究
Purification of tea saponin and its inhibition on tyrosinase activity
摘要
Abstract
Purification of tea saponin as well as inhibitory effect and inhibiting mechanism for catalytic synthesis of DOPA quinone from L-DOPA by potato tyrosinase in vitro was examined. The tea saponin product that purchased from market,was treated with macroporous resin to get the preliminarily refined tea saponin product (TS ). Then the TS were further purified by chromatographic method and two kinds of purified tea saponin product were produced as TS1 and TS2. Results show that at mass concentration of 100 g/L,the inhibition rate of the market product,the TS,TS1 and TS2 achieves 51. 29%,90. 06%,94. 13% and 82. 47%respectively. IC50 of TS and TS1 achieves 48 and 40 g/L respectively. The inhibition kinetics analyzed by Lineweaver-Burk plots show that the action of TS and TS1 express as reversible and competitive inhibition for conversion of L-DOPA. The inhibition constants for the TS and TS1 achieves 137 and 101 g/L respectively.关键词
美白化妆品添加剂/茶皂素/酪氨酸酶/抑制Key words
whitening cosmetic additive/tea saponin/tyrosinase/inhibition分类
化学化工引用本文复制引用
孙劲毅,蔡春辉,梁蓉,杨成..茶皂素的纯化及抑制酪氨酸酶活性的研究[J].日用化学工业,2017,47(6):312-316,340,6.基金项目
国家自然科学基金青年基金资助项目(31401533) （31401533）
