日用化学工业2017，Vol.47Issue(6)：317-321,5.DOI:10.13218/j.cnki.csdc.2017.06.004
茯苓提取物对酪氨酸酶抑制动力学及刺激性研究
Tyrosinase activity inhibition kinetics and skin irritation of Poria cocos extract
摘要
Abstract
The kinetics of inhibitory effect of Poria cocos extract on monophenolase and diphenolase activity of mushroom tyrosinase was studied. Test results show that Poria cocos extract efficiently inhibits both monophenolase and diphenolase activities of mushroom tyrosinase. Concentrations of Poria cocos extract leading to 50% activity lost (IC50 )on monophenolase and diphenolase were calculated to be 0. 008 and 0. 040 g/L respectively. There is a lag time in the catalytic reaction process of the monophenolase. Inhibition of Poria cocos extract on activity of diphenolase of tyrosinase indicates a reversible effect. Lineweaver-Burk plots indicates that the inhibitor binds the enzyme reversibly other than reacts with it destructively. Lineweaver-Burk plots show that the inhibition type is a composite of competitive and non-competitive inhibition. On this basis,Poria cocos extract stimulus preliminary assessment was conducted with RBC hemolysis test,and results show that Poria cocos extract with a mass concentration of 2. 000 g/L is non-stimulus while such concentration is far higher than IC50 of Poria cocos extract on monophenolase and diphenolase activity. It further indicates that Poria cocos extract displays whitening effect under the condition of the security. This suggests that Poria cocos extract with mass concentration range of 0. 200~0. 800 g/L is safe,for whitening cosmetic use.关键词
美白化妆品添加剂/茯苓提取物/酪氨酸酶/混合型抑制/刺激性Key words
additive of whitening cosmetics/Poria cocos extract/tyrosinase/mixed inhibition/stimulus分类
化学工程引用本文复制引用
陈宇霞,张凯,龚盛昭..茯苓提取物对酪氨酸酶抑制动力学及刺激性研究[J].日用化学工业,2017,47(6):317-321,5.基金项目
广东省高等职业院校珠江学者岗位计划资助项目(2013) （2013）
广东省高等学校高层次人才项目(2013QY02) （2013QY02）
2017年广州市科技计划项目产学研协同创新重大专项(201604016111) （201604016111）
