日用化学工业2017，Vol.47Issue(6)：326-330,335,6.DOI:10.13218/j.cnki.csdc.2017.06.006
透骨草抑菌活性成分提取及抑菌机理研究
Extraction for antibacterial active ingredient of Phryma leptostachya L. and its mechanism for bacteria inhibition
摘要
Abstract
Technological conditions for extraction of antibacterial active ingredient in Phryma leptostachya L. by ethanol was optimized. Then the extract was further extracted by a water immiscible organic solvent chloroform to enrich the effective antibacterial active ingredient (PAI). Extraction under the condition of ethanol volume fraction 80%,60 ℃,ratio of solid to liquid 1:30 (g:mL)and 4 h shows the optimal results,and the remaining aqueous phase raffinate after extraction with chloroform shows strong antibacterial activity. The diameter of inhibition zone for S. aureus and E. coli are (24. 32 ± 0. 65 )and (25. 44 ± 1. 10 )mm respectively,and the minimum inhibition concentration (MIC)are 6. 25 and 3. 12 g/L respectively. In addition,the bacterial growth curve,cell membrane permeability and cell morphology observation are applied to examine the inhibition mechanism on S. aureus and E. coli. Results show that PAI mainly effects on the logarithmic growth phase and destroys the permeability of the cell membrane as well as the structure of the cell wall;thus the bacteria are inhibited by multiple-hurdle effect.关键词
透骨草提取物/提取工艺/最小抑菌浓度/抑菌机理Key words
Phryma leptostachya L. extract/extraction process/minimum inhibition concentration/antibacterial mechanism分类
化学工程引用本文复制引用
张美龄,梁蓉,曹光群,杨成..透骨草抑菌活性成分提取及抑菌机理研究[J].日用化学工业,2017,47(6):326-330,335,6.基金项目
国家自然科学基金青年基金资助项目(31401533) （31401533）
