水产学报2017，Vol.41Issue(6)：827-835,9.DOI:10.11964/jfc.20170310748
水产加工副产物中磷脂和鱼油的分离及脂质组学研究
Separation of phospholipids and fish oil from fishery by-products followed by lipidomics study
摘要
Abstract
For full utilization of the fishery wastes, the aim of this study was to establish a method for extracting the phospholipids and fish oil simultaneously from wastes, followed by the analysis of phospholipid and fatty acid composition on the basis of multi-dimensional tandem mass spectrometry and gas chromatography. Phospholipids and fish oil were extracted simultaneously by modified Folch method and precipitation method with acetone. The extracting efficiency of five different extracts was compared, and the mixture of CH2Cl2-MeOH (1:2, V/V) performed best, whose extracting rates of phospholipids and fish oil were 0.97%和28.05%, respectively. Afterwards, the obtained fish oil was esterified and analyzed by gas chromatography, and the obtained phospholipids were injected directly by a syringe pump and identified and quantified by tandem mass spectrometry in the precursor-ion scan (PreIS) and neutral-loss scan (NLS) modes. The results show that fish oil contains 23 kinds of fatty acids, including oleic acid, linoleic acid, palmitic acid, etc. The content of unsaturated fatty acid is 75.97%. There are four classes (phosphatidyl choline, phosphatidyl ethanolamine, phosphatidyl inositol, phosphatidyl serine) and 69 kinds of phospholipid molecules were detected in the phospholipid extract. Phospholipids with at least one eicosapentaenoic acyl/docosahexaenoic acyl chain were frequently found, such as 16:0/20:5, 16:0/22:6, 18:1/22:6, 18:0/22:6, 20:0/22:6, etc. In conclusion, this method can efficiently extract phospholipids and fish oil simultaneously. It is simple, fast, and friendly to the environment.关键词
鱼油/磷脂/水产加工副产物/气相色谱法/串联质谱法/脂质组学Key words
fish oil/phospholipids/fishery by-products/gas chromatographic/tandem mass spectrometry/lipidomics分类
生物科学引用本文复制引用
崔益玮,李诗言,王珏,宋恭帅,王扬,戴志远,沈清..水产加工副产物中磷脂和鱼油的分离及脂质组学研究[J].水产学报,2017,41(6):827-835,9.基金项目
国家自然科学基金(31601542) （31601542）
浙江省自然科学基金(LQ16C200001) （LQ16C200001）
浙江省重点研发项目(2017C03041) National Natural Science Foundation of China (31601542) （2017C03041）
Natural Science Foundation of Zhejiang Province (LQ16C200001) （LQ16C200001）
Key Project of Research and Development of Zhejiang Province (2017C03041) （2017C03041）
