四川中医2017，Vol.35Issue(6)：52-55,4.
百诃清金颗粒对气道高反应性大鼠气道炎症的影响
Effect of Baihe Qingjin Granule on Airway Inflammation in Rats with Airway Hyperresponsiveness
摘要
Abstract
Objective:To observe the effect of Baihe Qingjin Granule on lung inflammation in rats with airway hyperresponsiveness.Methods:Forty-eight SD rats were randomly into 6 groups including the normal group,model group,dexamethasone group and three Baihe Qingjin Granule groups at dose of 21,10.5,5.25'g/kg with 8 rats in each groups.Copying the rats model of airway hyperresponsiveness which induced by Ovalbumin.According to the plan to gavage,recorded the total and differential leukocyte count in bronchoalveolar lavage fluid (BALF),detection of interleukin-4 (IL-4),interleukin-6 (IL-6) and interferon gamma (IFN-γ),tumor necrosis factor alpha (TNF-α) in BALF;observing the lung tissue pathological features under the HE staining.Results:Compared to the model group,Baihe Qingjin Granule in high and middle dose group can significantly reduce the white blood cells,eosinophils (P ＜ 0.001) and the number of neutrophils (P ＜ 0.05) in rats of BALF with AHR,and alleviated the inflammatory injury in lung tissue.Compared with the normal group,the levels of I.L-4,IL-6 and TNF -α in the model group were significantly increased (P ＜ 0.001),and the levels of IFN-γ was obviously decreased (P ＜ 0.001);The high dose group could significantly reduce the expression of IL-4 (P ＜ 0.001),IL-6,TNF-α (P ＜ 0.01),and increased the level of IFN-γ (P ＜ 0.01),which was similar to dexamethasone group.The levels of IL-4 and TNF-α in the middle and the low dose group were lower than those in the model group,but the difference was no statistically significant.Conclusion:Baihe Qingjin Granule can significantly inhibit airway inflammatory in rats with airway hyperresponsiveness and showes marked dose-effect relationship,which is the part of the mechanism of treatment of cough after infection.关键词
百诃清金颗粒/气道高反应性/气道炎症/实验研究Key words
Baihe Qingjin Granule/Airway hyperresponsiveness/Inflammation/Experimental research分类
医药卫生引用本文复制引用
明溪,钟晓君,解宇环,王妍,熊磊..百诃清金颗粒对气道高反应性大鼠气道炎症的影响[J].四川中医,2017,35(6):52-55,4.基金项目
国家中医药管理局重点学科中医儿科学资助项目(编号:XK201301) （编号:XK201301）
云南省教育厅科学研究基金项目(编号:2014Y240),云南省卫生厅内设机构研究课题(编号:2014NS319). （编号:2014Y240）
