山东农业科学2017，Vol.49Issue(6)：7-12,25,7.DOI:10.14083/j.issn.1001-4942.2017.06.002
洋葱AcPME基因的克隆及序列分析
Cloning and Sequence Analysis of AcPME Gene in Onion
摘要
Abstract
The sequence of AcPME gene was obtained through rapid amplification of cDNA ends (RACE) based on the sample of onion flower bud.The GenBank accession number was JF913196.1.The AcPME genomic DNA consisted of 2 786 bases with 4 exons and 3 introns.The cDNA length was 2 228 bp with a 2 001 bp ORF and encoded 666 amino acids.The bioinformatic analysis showed that the AcPME protein consisted of a signal peptide and transmembrane domain (SP/TM), a PMEI structural domain and a PME structural domain, which belonged to type I with a long N terminal PRO-region in the PME families.The amino acid homologous alignment and phylogenetic tree analysis exhibited that there were higher identities between AcPME and PME of homologous monocotyledons such as rice, maize and sorghum.The three-dimensional structure map showed AcPME protein had an obvious shallow cleft, 4 amino acid residues (T423, Q453, R565 and W567) binding to substrate and 2 residues (D476 and D497) involved in catalysis.The research laid a foundation for the biological functions of AcPME protein and the interactions between PMEI and PME domains.关键词
洋葱/果胶甲酯酶(PME)/克隆/序列分析Key words
Onion/Pectin methylesterase (PME)/Cloning/Sequence analysis分类
农业科技引用本文复制引用
孙亚玲,陈立,孙雨晴,杨妍妍,吴雄,曹辰兴,霍雨猛..洋葱AcPME基因的克隆及序列分析[J].山东农业科学,2017,49(6):7-12,25,7.基金项目
国家自然科学基金项目(31201635、31301786、31672165) （31201635、31301786、31672165）
山东省农业科学院青年英才重点扶持项目 （）
