山东农业科学2017，Vol.49Issue(6)：126-134,9.DOI:10.14083/j.issn.1001-4942.2017.06.027
基于16S rRNA基因的食源性致病菌实时荧光定量PCR检测研究
Real-Time Quantitative PCR Detection of Food-Borne Pathogens Based on 16S rRNA Gene
摘要
Abstract
In this study, a multiplex real-time quantitative PCR detection technology for the three food-borne pathogens, Shigella, Salmonella and Staphylococcus aureus, had been established utilizing the TaqMan probe and 16S rRNA gene as a target gene.The results indicated that the established multiplex real-time quantitative PCR detection system had good specificity and sensitivity, was simple and efficient, and greatly reduced the detection time, so it had a good prospect in the rapid screening for food-borne pathogens.关键词
食源性致病菌/16SrRNA基因/多重实时荧光定量PCR/TaqMan探针Key words
Food-borne pathogen/16S rRNA gene/Multiplex real-time quantitative PCR/TaqMan probe分类
轻工纺织引用本文复制引用
刘玲雪,孙红炜,李凡,徐晓辉,高瑞,杨淑珂,路兴波..基于16S rRNA基因的食源性致病菌实时荧光定量PCR检测研究[J].山东农业科学,2017,49(6):126-134,9.基金项目
山东省优秀中青年科学家科研奖励基金项目(BS2013NY012) （BS2013NY012）
