山东医药2017，Vol.57Issue(23)：20-23,4.DOI:10.3969/j.issn.1002-266X.2017.23.006
白花蛇舌草乙醇提取物对人肾癌细胞786-0体外增殖及小鼠肾癌细胞786-0移植瘤生长的影响
Effects of ethanol extracts of Hedyotis Diffusae Herba on in-vitro proliferation of human renal cancer 786-0 cells and growth of mouse renal cancer 786-0 cell tumor xenograft
摘要
Abstract
Objective To investigate the effects of ethanol extracts of Hedyotis Diffusae Herba (EEHDH) on in-vitro proliferation of human renal cancer 786-0 cells and growth of mouse renal cancer 786-0 cell tumor xenograft.Methods ①Renal cancer 786-0 cells were divided into the observation groups 1, 2, 3, 4, and the control group.Cells in the observation groups 1, 2, 3, and 4 were respectively added with the final concentrations of 10, 20, 40, and 80 mg/L EEHDH, while cells in the control group were added with the same volume of culture fluid.We measured the OD490 of 490 nm at 24, 48, and 72 h after culture by MTT.②Forty-two Kunming mice were randomly divided into 7 groups: the model group, the low-dose EEHDH group, medium-dose EEHDH group, high-dose EEHDH group, the cyclophosphamide group, medium-dose EEHDH combined with cyclophosphamide group, and control group with six in each group.Except that the mice in the control group were normally fed, the rest of 36 mice were injected with 0.2 mL tumor cell suspension subcutaneously at left hind leg axillae and weighed at 24 h.Mice in the model control group were given the same volume of drinking wate by gavage for 14 d.Mice in the low-, medium-, and high-dose EEHDH groups were given 5.0, 10.0, and 20.0 g/kg EEHDH, respectively, once a day, for 14 d.Mice in the cyclophosphamide group were given 20 mg/kg cyclophosphamide once every 2 days for 7 times.Mice in the medium-dose EEHDH combined with cyclophosphamide group were given 20 mg/kg CTX on day 1, 3, and 5, followed by 10.0 g/kg on day 6, once a day for 9 times.At 24 h after the last administration, we killed the mice to weigh and calculate the anti-tumor rate in each group.Then we separated the thymuses and spleens, weighed precisely, and calculated the thymus index and spleen index.Results ①Under the same conditions, the OD490 of renal cancer 786-0 cells in the observation groups 1, 2, 3, and 4 was lower than that in the control group (all P<0.05).The OD490 of renal cancer 786-0 cells was reduced with the increased duration of incubation and increased EEHDH dose (all P<0.05).②At the end of the experiment, the tumor weight in the model group, the low-dose EEHDH group, medium-dose EEHDH groups, high-dose EEHDH group, cyclophosphamide group, and medium-dose EEHDH combined with cyclophosphamide group was respectively (2.31±0.28), (1.94±0.22), (1.59±0.26), (1.52±0.23), (0.92±0.24), (0.86±0.23) g.The inhibitory rates of the low-dose EEHDH group, medium-dose EEHDH groups, high-dose EEHDH group, cyclophosphamide group, and medium-dose EEHDH combined with cyclophosphamide group were 16.02%, 31.22%, 34.26%, 60.20%, and 62.77%, respectively.The Q value of the median-dose EEHDH was 0.87.The mouse thymus index and spleen index of the model group were higher than those of the control group (both P<0.05).The mouse thymus index and spleen index of the thymophosphamide group were lower than those of the model group (both P<0.05).The spleen index of the low-, medium-, and high-doses EEHDH groups was higher than that of the model group (P<0.05), and no significant difference was found in the mouse thymus index as compared with that of the model group (P>0.05).The mouse thymus index and spleen index of the medium-dose EEHDH combined with cyclophosphamide group were higher than that of the cyclophosphamide group (both P<0.05).Conclusion EEHDH can significantly inhibit the proliferation of human renal carcinoma 786-0 cells in vitro and inhibit the growth of mouse renal cancer 786-0 cell transplantation tumor in vivo.through the enhancement of spleen function.关键词
白花蛇舌草/肾癌/免疫调节/抗肿瘤Key words
Hedyotis Diffusae Herba/renal carcinoma/immunoregulation/antitumor分类
医药卫生引用本文复制引用
张林超,赵俊峰,孙继建,潘世杰,高世玉,崔洪泉..白花蛇舌草乙醇提取物对人肾癌细胞786-0体外增殖及小鼠肾癌细胞786-0移植瘤生长的影响[J].山东医药,2017,57(23):20-23,4.基金项目
河南省中医药科学研究专项课题(2014ZY02050). （2014ZY02050）
