山东医药2017，Vol.57Issue(23)：24-26,3.DOI:10.3969/j.issn.1002-266X.2017.23.007
转染pcDNA-FLAG-EXOSC2质粒对宫颈癌HeLa细胞增殖与凋亡的影响
Effects of transfection of pcDNA-FLAG-EXOSC2 plasmid on proliferation and apoptosis of cervical cancer HeLa cells
赵春妍 1崔晓腾 1钱宝鑫 1任媛媛 1杨洁 1高星杰1
作者信息
摘要
Abstract
Objective To observe the effects of pcDNA-FLAG-EXOSC2 transfection on the proliferation and apoptosis of cervical cancer HeLa cells.Methods The pcDNA-FLAG-EXOSC2 recombinant plasmid was constructed.HeLa cells were transfected with pcDNA-FLAG-EXOSC2 plasmid (FLAG-EXOSC2 over-expression group) or pcDNA3.1 (+) plasmid (vector control group).Then, CCK-8 assay was performed to observe the cell proliferation of each group (the results were shown as OD450), while flow cytometry was utilized to measure the apoptotic rate.Results The OD450 values of the FLAG-EXOSC2 over-expression group and vector control group were 0.6652±0.05094 and 0.4436±0.03519 (both P<0.05).The apoptotic rates of the FLAG-EXOSC2 over-expression group and vector control group were 5.99%±0.37% and 7.57%±0.64%, respectively (both P<0.05).Conclusion Transfection of pcDNA-FLAG-EXOSC2 plasmid may significantly accelerate the proliferation and inhibit the apoptosis of HeLa cells.关键词
宫颈癌/外切体复合物2/pcDNA-FLAG-EXOSC2质粒/细胞增殖/细胞凋亡Key words
cervical carcinoma/exosome component 2/pcDNA-FLAG-EXOSC2 plasmid/cell proliferation/apoptosis分类
生物科学引用本文复制引用
赵春妍,崔晓腾,钱宝鑫,任媛媛,杨洁,高星杰..转染pcDNA-FLAG-EXOSC2质粒对宫颈癌HeLa细胞增殖与凋亡的影响[J].山东医药,2017,57(23):24-26,3.
