山东医药2017，Vol.57Issue(23)：27-30,4.DOI:10.3969/j.issn.1002-266X.2017.23.008
不同浓度小檗碱对OX-LDL诱导单核细胞源性树突状细胞分化成熟的影响
Effects of different concentrations of berberine on differentiation and maturation of human monocyte-derived dendritic cells induced by oxidized low-density lipoprotein
摘要
Abstract
Objective To investigate the influence of different concentrations of berberine on the differentiation and maturation of human monocyte-derived dendritic cells (Mo-DCs) induced by oxidized low-density lipoprotein (OX-LDL).Methods Human peripheral blood CD14+ monocytes were separated by magnetic activated cell sorting and were induced to differentiate into Mo-DCs by the recombinant human granulocyte-monocyte colony-stimulating factor and recombinant human interleukin-4.Mo-DCs were randomly divided into the experimental groups 1, 2, 3, the model group, and control group.After being added with OX-LDL at the final concentration of 50 ng/mL, Mo-DCs in the experimental groups 1, 2, and 3 were added with berberine at the final concentrations of 0.25, 0.75, and 1.5 μmol/L, respectively.The Mo-DCs in the model group were added with 50 ng/mL OX-LDL.Mo-DCs in the control group were given the same amount of PBS.They were all cultured for 48 h.The phenotypes of Mo-DCs (CD80, CD40, CD11c and HLA-DR) were detected by flow cytometry.The concentrations of interleukin-12 (IL-12) and interleukin-10 (IL-10) in the supernatant of Mo-DCs were detected by ELISA.Results Compared with the control group, the expression of CD80, CD40, and HLA-DR was significantly higher (all P<0.05);the concentration of IL-12 in Mo-DCs was higher (P<0.05) whereas the concentration of IL-10 was lower in the model group (P<0.05).Compared with the model group, the expression of CD80, CD40, and HLA-DR was significantly lower (all P<0.05);the concentration of IL-10 in Mo-DCs was higher whereas the concentration of IL-12 was lower in the all experimental groups (P<0.05).Compared with the experimental group 1, the expression of CD80, CD40, and HLA-DR was significantly lower (all P<0.05);the secretion of IL-10 on Mo-DCs was higher whereas IL-12 was lower in the experimental groups 2 and 3 (P<0.05).Compared with experimental group 2, the expression of CD80, CD40, and HLA-DR was significantly lower (all P<0.05);the concentration of IL-10 in Mo-DCs was higher whereas the concentration of IL-12 was lower in the experimental group 3 (P<0.05).Conclusion Berberine inhibits the immune maturation of human Mo-DCs induced by OX-LDL and this effect is dose dependent.关键词
小檗碱/树突状细胞/氧化低密度脂蛋白/动脉粥样硬化Key words
berberine/dendritic cells/oxidized low-density lipoprotein/atherosclerosis引用本文复制引用
李沫,李启伟,吴行贵,李有强..不同浓度小檗碱对OX-LDL诱导单核细胞源性树突状细胞分化成熟的影响[J].山东医药,2017,57(23):27-30,4.基金项目
广东省中医药局科研项目(20162053) （20162053）
广东省科技计划项目(2016ZC0122). （2016ZC0122）
