山东医药2017，Vol.57Issue(24)：16-19,后插1,5.DOI:10.3969/j.issn.1002-266X.2017.24.005
重复肢体远隔缺血适应对慢性脑低灌注大鼠空间学习记忆的影响及机制
Effect of repeated limb remote ischemia conditioning on spatial memory and learning in chronic cerebral hypoperfusion rats
摘要
Abstract
Objective To explore the effect and mechanism of repeated limb remote ischemic conditioning (LRIC) on spatial memory and learning in chronic cerebral hypoperfusion rats.Methods Sixty male SD rats were selected and randomly divided into the sham group, model group, single LRIC group, and multiple LRIC group.The rats in the latter three groups underwent bilateral carotid artery occlusion (2VO) to establish chronic cerebral hypoperfusion models, and the bilateral carotid arteries of rats in the control group were only separated without ligation.Three days after surgery, rats in the single LRIC group received LRIC just once, the detailed method was that ribbon gauzes were used to ligature hind limbs for occluding the femoral artery, ligating for 10 min, loosening for 10 min, and three circles each time.Rats in the multiple LRIC group received LRIC once a day for 6 w.Four weeks after LRIC was finished, Morris water maze test was used to test escape latency and target quadrant percentage, cerebral blood flow of hippocampal CA1 region was measured with laser Doppler, and the ultrastructure of axons and blood vessels in hippocampal CA1 region was observed by electron microscopic.Results Escape latency of the sham group, multiple LRIC group was shorter than that of the model group and single LRIC group, but the target quadrant percentage was higher than that of the model group and single LRIC group (P<0.05 or P<0.01).The relative cerebral blood flow of hippocampal CA1 region in the sham group, model group, single LRIC group, and multiple LRIC group was 100%, 61.3%±11.7%, 59.5%±13.1%, and 80.2%±14.6%, respectively.The relative cerebral blood flow of the sham group and multiple LRIC group was higher than that of the model group and single LRIC group (all P<0.05).Endothelium was not smooth;thickening of the basement membrane, obvious peripheral edema, axonal fracture, and plate separation were observed in CA1 region of the model group;the changes mentioned above alleviated significantly in the multiple LRIC group (blood vessels and axons changes were not tested because there was no significant difference in spatial learning memory and cerebral blood flow between the single LRIC group and model group).Conclusion Repeated LRIC can improve cognitive impairment caused by chronic cerebral blood flow hypoperfusion by increasing blood supply in hippocampus and inhibiting microvascular injury in hippocampus.关键词
慢性脑低灌注/肢体远隔缺血适应/Morris水迷宫试验/大鼠Key words
chronic cerebral hypoperfusion/limb remote ischemic conditioning/Morris water maze test/rats分类
医药卫生引用本文复制引用
张安波,李宁,韩荣荣,任长虹..重复肢体远隔缺血适应对慢性脑低灌注大鼠空间学习记忆的影响及机制[J].山东医药,2017,57(24):16-19,后插1,5.基金项目
国家自然科学基金资助项目(81573867) （81573867）
首都卫生发展科研专项资助项目(首发 2011-1001-03). （首发 2011-1001-03）
