水生生物学报2017，Vol.41Issue(4)：735-740,6.DOI:10.7541/2017.91
基于简化基因组测序的大黄鱼耐高温性状全基因组关联分析
GENOME-WIDE ASSOCIATION STUDY OF THERMAL TOLERANCE IN LARGE YELLOW CROAKER LARIMICHTHYS CROCEA BASED ON SLAF-SEQ TECHNOLOGY
摘要
Abstract
Twenty thermal-tolerant and twenty thermal-sensitive individuals ofLarimichthys crocea were sequenced using specific-locus amplified fragment (SLAF-seq) technology based on Illumina HiSeqTM2500 platform. 419211 SN-Ps were identified with an average read depth of 10.26× for each sample. Thirty-eight SNPs (P<2.39E–08) signifi-cantly related with thermal tolerance trait were identified according to association analysis. The SNP locations in large yellow croaker genome were identified using BLAST program, and functional genes around SNP were annotated. Twenty-six genes with known functions were discovered around 38 SNPs, which mainly regulate cell transcription, metabolism and immunity. These results provide basic information to analyze thermal-tolerant molecular mechanism and develop thermal-tolerant lines ofLarimichthys crocea in the future.关键词
大黄鱼/高温胁迫/简化基因组测序/单核苷酸多态性/全基因组关联分析Key words
Larimichthys crocea/High temperature/SLAF-seq/SNP marker/GWAS分类
生物科学引用本文复制引用
陈小明,李佳凯,王志勇,蔡明夷,韩芳,刘贤德..基于简化基因组测序的大黄鱼耐高温性状全基因组关联分析[J].水生生物学报,2017,41(4):735-740,6.基金项目
国家自然科学基金(31172397和31402339) （31172397和31402339）
福建省高等学校新世纪优秀人才支持计划(JA14167)资助[Supported by the National Natural Science Foundation of China (31172397, 31402339) （JA14167）
the New Century Excellent Talents of Fujian Province University (JA14167)] （JA14167）
