饲料中添加合成虾青素对中华绒螯蟹成体雌蟹性腺发育、色泽和抗氧化能力的影响
EFFECTS OF DIETARY SUPPLEMENTATION OF SYNTHETIC ASTAXANTHIN ON OVARIAN DEVELOPMENT, COLORATION AND ANTIOXIDANT CAPACITY OF ADULT FEMALE CHINESE MITTEN CRAB, ERIOCHEIR SINENSIS
摘要
Abstract
To investigate the effects of dietary supplementation of synthetic astaxanthin on ovarian development, colora-tion and antioxidant capacity of adult female Chinese mitten crab (Eriocheir sinensis), three isonitrogenous and isoli-pidic fattening diets with 42% crude protein and 16% crude lipid were formulated by adding 0, 130 and 260 mg/kg syn-thetic astaxanthin (named as Diet 1, Diet 2, and Diet 3, respectively), and commercial fattening diet was used (named as Diet 4). Each diet was fed to triplicate tanks with 12 female crabs per tank for 60-day fattening. The results showed that dietary supplementation of synthetic astaxanthin had no significant effect on the hepatosomatic index (HSI) and gon-adosomatic index (GSI) of adult femaleE. sinensis (P>0.05). Diet 3 treatment had the highest contents of carotenoid and redness value (a), and Diet 1 group had the highest lightness value (L) and yellow value (b) in the ovaries. The highest and the lowestb value of carapace were found in Diet 2 and Diet 1 treatments, respectively (P<0.05). Diet 1 group had the highest activity of peroxidase (POD) in the serum. The activities of catalase (CAT), glutathione re-ductase (GR), lactic dehydrogenase (LDH), total antioxidant capacity (T-AOC) and the contents of malondialdehyde (MDA) and lactic acid (LD) in serum from Diet 4 group were significantly higher than those of other groups (P<0.05). The highest and lowest activities of superoxide dismutase (SOD), T-AOC, glutathione peroxidase (GSH-Px) and GR in hepatopancreas were found in Diet 1 treatment and Diet 2 treatment, respectively (P<0.05). The activity of acid phos-phatase (ACP) and hemocyanin (Hc) content in the serum from Diet 2 treatment were signifi-cantly higher than those of other treatments (P<0.05). The highest activities of alkaline phosphatase (ALP) and γ-glutamyl transpeptidase (γ-GT), and nitric oxide (NO) content were found in Diet 4 treatment. The highest activities of ACP, ALP and γ-GT in the hep-atopancreas were shown in Diet 1 group. In conclusion, dietary supplementation of synthetic astaxanthin did not signi-ficantly affect the ovarian development of adult female crabs, but could enhance content of carotenoid, coloration and antioxidant capacity. These results suggest that the appropriate content of synthetic astaxanthin in the fattening diet is 90 mg/kg for femaleE. sinensis.关键词
合成虾青素/中华绒螯蟹/雌体/性腺发育/色泽/抗氧化能力Key words
Synthetic astaxanthin/Eriocheir sinensis/Female/Ovarian development/Coloration/Antioxidant capacity分类
农业科技引用本文复制引用
麻楠,龙晓文,赵磊,常国亮,吴旭干,成永旭..饲料中添加合成虾青素对中华绒螯蟹成体雌蟹性腺发育、色泽和抗氧化能力的影响[J].水生生物学报,2017,41(4):755-765,11.基金项目
科技部港澳台科技合作专项项目(2014DFT30270) （2014DFT30270）
上海市科学技术委员会科研项(13320502100, 16DZ2281200) （13320502100, 16DZ2281200）
上海高校水产学一流学科建设项目(2012-62-0908) （2012-62-0908）
深圳澳华农牧科技有限公司委托项目(D-8006-15-0054) （D-8006-15-0054）
江苏省科技厅政策引导类产学研合作项目(BY2016062)资助[Supported by the Hongkong, Macao and Taiwan Science and Technology Cooperation Projects (2014DFT30270) （BY2016062）
the Projects (13320502100 and 16DZ2281200) from Shanghai Municipal Science and Technology Commission （13320502100 and 16DZ2281200）
the Shanghai Universities First-class Disciplines Project of Fisheries (No.2012-62-0908) from Shanghai Municipal Education Committee （No.2012-62-0908）
Technology Commission and the R&D Project (D-8006-15-0054) from Shenzhen Alpha Feed Co. Ltd and Jiangsu Department of Science and Technology Policy Guidance Research Cooperation Project (BY2016062)] （D-8006-15-0054）
