水生生物学报2017，Vol.41Issue(4)：793-799,7.DOI:10.7541/2017.99
两株副溶血弧菌烈性噬菌体的分离鉴定
ISOLATION AND IDENTIFICATION OF TWO LYTIC PHAGES AGAINST VIBRIO PARAHAEMOLYTICUS
摘要
Abstract
The aim of this study was to screen lytic phase and provide new alternatives for the prevention and control of V. parahaemolyticus. In this study, two lytic phages SX-2 and SX-F were isolated by double-layer plate method against the host bacteriaV. parahaemolyticus Vp13. The morphological structures of these two phages were observed by trans-mission electron microscope (TEM). The types of nucleic acids were identified by the degradation characteristics of DNase I,RNase A,Mung Bean Nuclease andHind Ш. The lysis spectrum, optimal multiplicity of infection and one step growth curve of SX-2 and SX-F were also analyzed. The results of TEM showed that the capsid protein of lytic phage of SX-2 was a complex symmetry with icosahedral symmetry head that had length of about 110 nm and width of about 50 nm and a spiral symmetry tail that had length of about 110 nm and width of about 10 nm. SX-F showed hexagon with length of about 56.86 nm and width of about 50.74 nm without a tail, which was icosahedral symmetry. Both SX-2 and SX-F were linear double stranded DNA bacteriophage. They were belonged to the family ofMyoviri-dae andTectivirus based on the taxonomy standard of the ninth report of the International Committee Taxonomy of Viru-ses. Results of lytic experiment on 85Vibrio strains showed that phage SX-2 could lysis 23 strains ofV. parahaemolyti-cus and one strain ofVibrio alginolyticus, and phage SX-F could lysis 19 strains ofV. parahaemolyticus and one strain ofV. alginolyticus. The optimal multiplicity of infection of plase SX-2 and SX-F were 0.0001. One step growth curve showed that the incubation period of phage SX-F was about 10min, the lytic cycle was 70min, and the burst size was 116.2. The incubation period of the phage SX-2 was less than 10min, and the lytic cycle was about 70min, and the burst size was 209.3. These results indicated that both of them were lytic phages, which can be used as an alternative to con-trol and prevention of theV. parahaemolyticus.关键词
副溶血弧菌/烈性噬菌体/形态结构/生物学特性Key words
Vibrio parahaemolyticus/Lytic phage/Morphological structure/Biological characteristics分类
农业科技引用本文复制引用
宋增福,徐华东,彭孟凡,孙博超,赵政,张也,任建峰,张庆华..两株副溶血弧菌烈性噬菌体的分离鉴定[J].水生生物学报,2017,41(4):793-799,7.基金项目
上海市水产遗传育种协同创新中心基金(ZF1206) （ZF1206）
上海海洋大学博士启动基金(A2-0203-00-100319) （A2-0203-00-100319）
上海市高校新进教师培训及科研启动基金(A1-2056-16-0021)资助[Supported by the Shanghai Collaborative Innovation Center for Aquaculture Genetics and Breeding (ZF1206) （A1-2056-16-0021）
Shanghai Ocean University Doctor start-up Fund(A2-0203-00-100319) （A2-0203-00-100319）
Shanghai New College Teacher Training and Research Start-up Fund(A1-2056-16-0021)] （A1-2056-16-0021）
