水生生物学报2017，Vol.41Issue(4)：800-806,7.DOI:10.7541/2017.100
鱼源无乳链球菌的血清型及分子分型研究
SEROTYPES AND MOLECULAR TYPING OF STREPTOCOCCUS AGALACTIAE ISOLATED FROM FISH
摘要
Abstract
To analyze molecular typing characteristics ofStreptococcus agalactiae isolated from different fish sources and understand the correlation and homology between the strains, this study identified capsular polysaccharide sero-type (CPS) with multiplex PCR for 26S. agalactiae. Multilocus sequence typing (MLST) and pulsed field gel electro-phoresis (PFGE) were adopted to study the molecular characteristics and homology based on the specificcfb gene ofS. agalactiae. The results showed that CPS type had Ia (n=22) and III (n=4) two types, of whichS. agalactiae isolated fromSchizopygopsis pylzovi and tilapia were Ia, andS. agalactiae isolated fromSchizothorax prenati existed Ia (n=2) and III (n=4) two types. Multilocus sequence typing received three STs sequence types (ST-891、ST-103、ST-19), among which the sequence types ofS. agalactiae isolated fromSchizopygopsis pylzovi and tilapia were new sequence type ST-891, andS. agalactiae isolated fromSchizothorax prenati existed ST-19 and ST-103 two sequence types. PFGE cluster analysis created 16 banding patterns (A-P), and the cluster P was the preponderant type. Similar capsular polysaccharide serotype-MLST type strains presented high concentration in the PFGE type. CPS types, MLST types and PFGE types were correlated, but CPS types, STs sequence types and PFGE banding types had no obvious correla-tion with the host's sources. The similarity of molecular characteristics ofS. agalactiae isolated from different fish sources suggests the possibility of cross infection, and the diversity of molecular characteristics ofSchizothorax prenati suggest the existence of heritable variation.关键词
无乳链球菌/荚膜多糖血清分型/多位点序列分型/脉冲场凝胶电泳Key words
Streptococcus agalactiae/Capsular polysaccharide serotype/Multilocus sequence typing/Pulsed Field Gel Electrophoresis分类
农业科技引用本文复制引用
张雨薇,耿毅,余泽辉,汪开毓,陈德芳,向正刚,李亚军,郭坤宁,牟维豪..鱼源无乳链球菌的血清型及分子分型研究[J].水生生物学报,2017,41(4):800-806,7.基金项目
四川省淡水鱼产业技术体系创新团队建设项目资助[Supported by Sichuan Province Freshwater Fish Industry System Innovation Building Project Funding] （）
