水生生物学报2017，Vol.41Issue(4)：904-913,10.DOI:10.7541/2017.113
硫素营养水平对产油尖状栅藻光合生理及生化组成的影响
EFFECTS OF SULFUR CONCENTRATION ON THE PHOTOSYNTHETIC PHYSIOLOGY AND BIOCHEMICAL COMPOSITION OF SCENEDESMUS ACUMINATUS
摘要
Abstract
Scenedesmus acuminatus microalgae are capable of accumulating lipids when exposed to different sulfur concentrations and are therefore considered promising organisms for biodiesel production. Using column photobiorea-ctors, the relevance of different sulfur concentration (2.0S, 1.0S, and 0.25S) on the growth and photosynthetic physiology of the algae was investigated. The initial sulfur concentration had a significant effect on the growth ofS. acuminatus (P<0.05). A maximum biomass of 7.47 g/L was obtained in the 2.0S group, which was significantly higher than that of the 1.0S and 0.25S groups (6.43 g/L and 4.17 g/L, respectively;P<0.05), indicating that the addition of sul-fur-rich nutrients could promote algae growth. The changes in chlorophyll and band total carotenoid content inS. acuminatus positively correlated with the initial sulfur level in the medium. At the beginning of cultivation, rapid accu-mulation of carbohydrates occurred under low sulfur conditions; the 0.25S group achieved the highest carbohydrate content accounting for 44.37% of the dry weight, which was 14.43% and 13.78% higher than that of the 1.0S and 2.0S groups, respectively. With a decreased carbohydrate and protein content, lipid content increased significantly in the later stages of cultivation. The 0.25S group acquired the maximum lipid content (55.15% of the dry weight), which was significantly higher than that of the other two groups (P<0.05). Meanwhile, the photosynthetic oxygen evolution rate, maximum light energy conversion efficiency of PSⅡ (Fv/Fm), actual light energy conversion efficiency (yield), and the relative electron transfer efficiency (ETR) positively correlated with the initial sulfur concentration in the culture medium;the whole culture period showed a tendency to increase first and then significantly decrease. The results of fluores-cence emission spectroscopy at 77 K showed that there was light energy allocation between the two photosynthetic sys-tems at the cultivation prophase. In conclusion, growth, lipid accumulation, and photosynthetic physiology ofS. acuminatus were apparently influenced by the sulfur concentration.关键词
尖状栅藻/硫素营养/油脂积累/光合生理参数Key words
Scenedesmus acuminatus/Sulfur Concentration/Lipid accumulation/Photosynthetic physiological parameter分类
农业科技引用本文复制引用
王倩雅,张莹,李爱芬,张成武..硫素营养水平对产油尖状栅藻光合生理及生化组成的影响[J].水生生物学报,2017,41(4):904-913,10.基金项目
国家自然科学基金(41176105) （41176105）
中央高校基本科研业务费专项资金(21614101)资助[National Natural Science Foundation of China (41176105) （21614101）
Fundamental Research Funds for the Central Universities (21614101)] （21614101）
