生态学报2017，Vol.37Issue(11)：3636-3646,11.DOI:10.5846/stxb201603220512
施氮肥对华北平原土壤氨氧化细菌和古菌数量及群落结构的影响
Effects of nitrogen fertilizer application on abundance and community structure of ammonia oxidizing bacteria and archaea in a North China agricultural soil
摘要
Abstract
The abundance and community structure of total bacteria ammonia oxidizing bacteria,and archaea were investigated by real-time PCR,T-RFLP,and cloning library approaches in a wheat field under different N fertilization rates (no N fertilization,0 kg N/hm2,CK;low N fertilization rate,75 kg N/hm2,N1;moderate N fertilization rate,150 kg N/hm2,N2;high N fertilization rate,225 kg N/hm2,N3) in North China.The population sizes of total bacteria,AOB and AOA were 5.74× 109-7.50× 109,8.89× 106-2.66× 107 and 3.83× 108-7.78× 108 copies/g dry soil,respectively.The population numbers of AOA were higher than that of AOB in all treatments,with AOA to AOB ratios ranged from 81.72 to 14.38.Significant higher numbers for AOB were detected for the high level of N fertilization soil (P＜0.05),but not in the population of total bacteria and AOA.The population numbers of AOB observed in N1,N2 and N3 were 1.64,2.50 and 2.99 times greater than that in the CK treatment,respectively.The N fertilization rates significantly changed the communities of both AOB and AOA,and more variation was observed in the community of AOB.Phylogenetic results showed that the majority AOB sequences fell into two branches of Cluster 3,affiliated with Nitrosospira species,and all AOA sequences fell within four branches in Cluster S.Significant positive correlations were observed among the population sizes of AOB to total nitrogen and ammonium,and the population sizes of AOA to soil pH and nitrate (P＜0.05).Significantnegative correlations were observed among the population sizes of AOB to soil pH and C/N ratio,and the population sizes of AOA to ammonium (P＜0.05).These results demonstrated that AOB were more sensitive than AOA to N fertilization in alkaline wheat fields in North China.关键词
氮肥/氨氧化细菌/氨氧化古菌/丰度/群落结构/末端限制性片段长度多样性Key words
nitrogen fertilizer/ammonia oxidizing bacteria (AOB)/ammonia oxidizing archaea (AOA)/abundance/community structure/terminal restriction fragment length polymorphism (T-RFLP)引用本文复制引用
杨亚东,张明才,胡君蔚,张凯,胡跃高,曾昭海..施氮肥对华北平原土壤氨氧化细菌和古菌数量及群落结构的影响[J].生态学报,2017,37(11):3636-3646,11.基金项目
公益性行业(农业)科研专项(201503121-11) （农业）
河北省科技攻关项目(14227008D) （14227008D）
