生物骨科材料与临床研究2017，Vol.14Issue(3)：5-8,后插1-后插2,6.DOI:10.3969/j.issn.1672-5972.2017.03.002
12/15-脂氧合酶抑制剂黄芩素对软骨细胞凋亡的抑制作用研究
Inhibition of 12/15 lipoxygenase by baicalein alleviates chondrocyte apoptosis
摘要
Abstract
Objective To investigate the inhibitory effect and possible pathogenesis of Baicalein,12/15-lipoxygenase inhibitor,on apoptosis of chondrocytes induced by sodium nitroprusside (SNP).Methods Control group,SNPgroup and Baicalein group were setting.The 0.5 mM SNP was used to induce apoptosis for 24 hours,meanwhile,Baicalein group was treated additionally with a variety of concentration gradient of 5 μM,25 μM,50 μM,100 μM to determine the optimum concentration.The control group received only the same volume of solvent.The cytotoxicity of reagents was detected by CCK8.The Annexin V/PI double staining was used to determine the apoptosis rate.The immunofluorescence was used to detect the intracellular localization of apoptosis inducing factor (AIF) in chondrocytes.Results Baicalein has no statistically significant in toxicity to chondrocytes at multiple concentration gradients,the activity of chondrocytes in the SNP group decreased significantly compared with the control group (P＜0.01),and Baicalein increased the cytoactive in a concentration-dependent manner compared with the SNP group (P＜0.01),flow cytometry analysis showed that the early apoptosis rate of chondrocytes was 3.16％ in control group,27.8％ in SNP group and 14.1％ in 100 μM Baicalein group,respectively.Immunofluorescence assay showed that the nuclear translocation of AIF was observed in SNP group compared with the control group,while the nuclear translocation of AIF was inhibited in Baicalein-treated group.Conclusion The 12/15-lipoxygenase inhibitor,Baicalein,has anti-apoptosis effect on chondrocytes through inhibiting the release of AIF from mitochondria and nuclear translocation.关键词
花生四烯酸盐12-脂氧合酶/花生四烯酸盐15-脂氧合酶/脂氧合酶抑制剂/软骨细胞/细胞凋亡Key words
Arachidonate 12-Lipoxygenase/Arachidonate 15-Lipoxygenase/Chondrocytes/Apoptosis分类
生物科学引用本文复制引用
陈恺哲,燕宇飞,袁俊,郭蕾,何川..12/15-脂氧合酶抑制剂黄芩素对软骨细胞凋亡的抑制作用研究[J].生物骨科材料与临床研究,2017,14(3):5-8,后插1-后插2,6.基金项目
上海市卫生和计划生育委员会科研基金项目(201540275) （201540275）
上海市中西医结合临床重大项目(ZY3-LCPT-2-2003) （ZY3-LCPT-2-2003）
