生物技术通报2017，Vol.33Issue(7)：100-106,7.DOI:10.13560/j.cnki.biotech.bull.1985.20170002
氮肥对甘蔗叶片内生固氮菌nifH基因表达的影响
Effects of Nitrogen Fertilizer on the Expression of nifH Gene of Endophytic Azotobacter in Sugarcane Leaves
摘要
Abstract
In order to investigate the effects of nitrogen fertilizer on the expression of nifH gene of endophytic diazotrophs in sugarcane leaves,we carried out a field experiment with and without nitrogen fertilization,and used RT-PCR and Western blot technology to analyze the differences of nifH expression and the relative expression quantity of nitrogenase in 3 sugarcane varieties,GXB9,ROC22 and GT11. The results showed that the expressions of nifH gene in endogenous azotobacter were detected at gene and protein levels in the leaves of sugarcane growing in field both with nitrogen fertilization and without nitrogen fertilization. Nitrogen fertilization treatment inhibited or decreased the expression of nifH gene in nitrogen-fixing bacteria,but it was beneficial to the expression of nifH at the protein level. The relative expressions of the nifH gene in the leaves of three sugarcane cultivars were all higher under nitrogen application than the control without nitrogen application ;especially,the difference was significant for cultivar ROC22.关键词
甘蔗/内生固氮菌/固氮酶/nifH基因Key words
sugarcane/endogenous azotobacter/nitrogenase/nifH引用本文复制引用
李佳慧,袁丹,陆建明,杨丽涛,李杨瑞,邢永秀..氮肥对甘蔗叶片内生固氮菌nifH基因表达的影响[J].生物技术通报,2017,33(7):100-106,7.基金项目
国家自然科学基金项目(3156100093、31471449),广西八桂学者和特聘专家专项基金(2013),国家现代农业产业技术体系广西甘蔗创新团队项目(gjnytxgxcxtd-03),广西农业科学院团队项目(2015YT02) （3156100093、31471449）
