山西农业大学学报（自然科学版）2017，Vol.37Issue(6)：402-407,6.
几种药用植物内生细菌对苹果腐烂病菌(Valsa mali)抑菌效果研究
Study on the bacteriostatic effect of endophytic bacteria in several medicinal plants to Valsa mali
摘要
Abstract
[Objective]In order to clearing the antibacterial effect of the 13 endophytic bacteria isolated from medicinal plant to V.mali.[Methods]we studied the inhibition effect of live bacteria and fermentation broth of 13 endophytic bacteria to V.mali by using pair culture method and plate growth rate method.13 endophytic bacteria could be divided into two types according to the cluster analysis of inhibitory rate of live bacteria.[Results]The first type included DS-1,CJ-2,DS-3,ZY-1,MY-1,DS-9,MH and MY-4,whose inhibitory rate was in the range of 81.88% to 83.65%.The second type included ZJ-2,L-23,ZJ-1,ZJ-3 and CJ-1,whose inhibitory rate was in the range of 79.76% to 81.29%.13 strains could be divided into two types according to the cluster analysis of inhibitory rate of the fermentation broth.The first type included MY-1、MY-4、CJ-1、CJ-2、ZJ-1、DS-3 and ZJ-3 and the range of their inhibitory rate was 60.00% to 81.53%.The second type included ZJ-2、MH、DS-9、L-23、ZY-1 and DS-1,whose inhibitory rate was in the range of 12.94% to 43.24%.The cluster analysis of the inhibitory rate of the live bacteria and fermentation broth showed that the strains could be divided into four groups and the first group was the optimal group with the strongest antibacterial effect from 68.29% to 83.65%.This group included all the live bacteria of the 13 antagonistic bacterial and the fermentation broth of MY-4 and MY-1.[Conclusion]Therefore,we could develop the live bacteria and fermentation broth of MY-1,MY-4 and the live bacteria of the other 11 bacteria to prevent the apple canker (V.mali).关键词
药用植物/内生细菌/腐烂病/黑腐皮壳菌/抑菌效果Key words
Medicinal plant/Endophytic bacteria/Apple canker/Valsa mali/Bacteriostatic effect分类
农业科技引用本文复制引用
郭薇,殷辉,周建波,吕红,张志斌,赵晓军,刘慧平,韩巨才..几种药用植物内生细菌对苹果腐烂病菌(Valsa mali)抑菌效果研究[J].山西农业大学学报（自然科学版）,2017,37(6):402-407,6.基金项目
山西省重点研发计划项目(201603D221013-3) （201603D221013-3）
山西省青年科技研究基金(面上青年基金项目)(201601D202073) （面上青年基金项目）
