陕西医学杂志2017，Vol.46Issue(7)：819-822,4.DOI:10.3969/j.issn.1000-7377.2017.07.001
肝X受体激动剂GW3965对高糖所致心肌细胞凋亡的影响及作用机制
The effect of liver X receptors agonist GW3965 on myocardial apoptosis induced by high glucose and its mechanism
摘要
Abstract
Objective: To explore the effect of GW3965 on myocardial apoptosis induced by high glucose and its mechanism.Methods: H9c2 myocardial cells were randomly divided into three groups: control group, high glucose group and GW3965 group.Cells in GW3965 group were pretreated with 10μmol/L GW3965 for 24 h.Then cultures in high glucose group and GW3965 group were induced by 33 mmol/L glucose for 24 h.MTT was used to detect cells survival.The apoptosis was detected by flow cytometry and mitochondrial membrane potential by JC-1 fluorescence staining.Western blot was used to detect the expression of proteins related to LXR, ERK and p38 MAPK signal pathway.Results: GW3965 could inhibit the apoptosis of H9c2 cells, increase the rate of cells survival and influence mitochondrial membrane potential.GW3965 inhibited the activity of ERK and p38 MAPK pathway.Conclusion: GW3965 can inhibit the apoptosis and mitochondrial damage induced by high glucose via regulating LXRα and MAPK pathway, resulting a protective effect on myocardial cells.关键词
心肌/@GW3965/高血糖症/糖尿病心肌病/病理生理学/@肝X受体/细胞凋亡Key words
Myocardium/@GW3965/Hyperplycemia/Diabetic cardiomyopathies/physiopathology/@Liver X receptors/Apoptosiss分类
医药卫生引用本文复制引用
成永霞,张大伟,孙立新,冯玉宽,郭素芬,刘贵波..肝X受体激动剂GW3965对高糖所致心肌细胞凋亡的影响及作用机制[J].陕西医学杂志,2017,46(7):819-822,4.基金项目
国家青年基金资助项目(81500629) （81500629）
