实用医学杂志2017，Vol.33Issue(9)：1373-1376,4.DOI:10.3969/j.issn.1006-5725.2017.09.003
STIM1对乳腺癌细胞移动的作用及机制
The mechanism research of STIM1 in breast cancer cells
摘要
Abstract
Objectives To explore the calcium signaling mechanism of STIM1 in breast cancer cells. Meth-ods After SiRNA interruption, Western blot and Transwell were used to measure protein expression of STIM1 and cell migration in MDA-MB-231 cells respectively. The relationship between STIM1 and SOCE calcium signaling were analysed by Laser confocal microscopy. Western blots were used to measure protein expression of FAK after si-lence STIM1. Results The numbers of cells without STIM1 were significantly lower than those cells with STIM1 by Transwell assay. STIM1 mediated SOCE in MDA-MB-231. Blocking SOCE might inhibite cells migration. Si-lence STIM1 did not affect the expression or activation of FAK in MDA-MB-231 cells. Conclusion STIM1 influ-ences cell migration through SOCE pathway in breast cancer cells, which is independent on the expression or activa-tion of FAK.关键词
乳腺癌细胞/STIM1/钙信号/FAKKey words
Breast cancer cells/STIM1/Calcium signaling/FAK引用本文复制引用
吴冰,林天骥,阮仕娟,王斌,邹飞..STIM1对乳腺癌细胞移动的作用及机制[J].实用医学杂志,2017,33(9):1373-1376,4.基金项目
国家自然科学基金(编号:81101611) （编号:81101611）
