实用医学杂志2017，Vol.33Issue(9)：1394-1396,3.DOI:10.3969/j.issn.1006-5725.2017.09.008
姜黄素通过降低MT1-MMP表达抑制乳腺癌细胞的增殖与侵袭
Inhibition effect of curcumin on proliferation and invasion of breast cancer cell by decreasing expression of MT1-MMP
余俊青 1姚广裕 1胡晓磊 1陈路嘉 1叶长生1
作者信息
- 1. 510515 广州市,南方医科大学南方医院乳腺科
- 折叠
摘要
Abstract
Objective To investigate whether MT1-MMP is involved in the inhibition effect of curcumin on the proliferation and invasion of breast cancer cell and the mechanism . Methods Firstly, MCF-7 cell lines transfected by MT1-MMP eukaryotic expression vector was established. We divided all cells into 3 groups,including null vector transfection group, non-transfected and transfected group with different concentrations of curcumin. The expression of MT1-MMP protein, the proliferation and invasion ability were respectively analyzed by western blot, transwell method, and cell counting kit-8 (CCK-8). Results The expression of MT1-MMP was inhibited by curcumin. Transwell and CCK-8 experiment indicated the proliferation and invasion abilities of MT1-MMP transfected MCF-7 cells were inhibited by curcumin in a concentration dependent manner. Conclusion The inhibition value of curcumin on proliferation and invasion is probably due to its ability to inhibit the expression of MT1-MMP.关键词
乳腺肿瘤/姜黄素/MCF-7细胞/MT1-MMPKey words
Breast neoplasm/Curcumin/MCF-7 cell/MT1-MMP引用本文复制引用
余俊青,姚广裕,胡晓磊,陈路嘉,叶长生..姜黄素通过降低MT1-MMP表达抑制乳腺癌细胞的增殖与侵袭[J].实用医学杂志,2017,33(9):1394-1396,3.
